Change of quantal size and parameters of release with stimulation in bovine chromaffin cells

Pflügers Archiv : European journal of physiology
M I Glavinović, J M Trifaró

Abstract

Changes in quantal size and in the parameters of release were examined in chromaffin cells using amperometric recordings during and following various stimuli that induce secretion. As a general rule, a greater quantal content was associated with a greater quantal size. Following a short depolarizing pulse (0.5-2 s; 100 mV from a holding potential of -80 mV), the mean value of quantal size increased by 54% over several seconds before gradually (over tens of seconds) returning toward the control value, whilst its variability rose by 62%. The changes observed following 30-s applications of high extracellular K+ (50 mM) were more modest. A rapid application of short depolarizing pulses (2 s every 10-20 s; 100 mV from a holding potential of -80 mV) also led, at least initially, to greater quantal content and quantal size. Mean quantal size rose initially by 68%, but decreased subsequently to 31% below pre-stimulation levels. In whole-clamped cells, the frequency of quantal release can rise abruptly, probably owing to a mechanical disturbance that makes the membrane leaky to Ca2+. In such cases, a marked rise in quantal content (>ten-fold) was paralleled by an almost as dramatic (up to ten-fold) rise in quantal size and an important,...Continue Reading

Citations

Feb 9, 2005·Journal of Neuroscience Methods·S KebirM I Glavinović
Nov 18, 2005·Journal of Neuroscience Methods·D GermainM I Glavinovic
Apr 16, 2009·Biochimica Et Biophysica Acta·M Tajparast, M I Glavinović
Jun 17, 2015·Biochimica Et Biophysica Acta·Mohammad TajparastMladen I Glavinović

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