Changes in angiopoietin expression in glomeruli involved in glomerulosclerosis in rats with daunorubicin-induced nephrosis

Acta Pharmacologica Sinica
Yuanhang LuJianshe Liu

Abstract

To study the potential pathological role of endogenous angiopoietins in daunorubicin-induced progressive glomerulosclerosis in rats. Seventy male Wistar rats were allocated randomly into a daunorubicin group (DRB; n=40) or a control group (n=30). The rats in the DRB group were injected with DRB (15 mg/kg), in their tails. Subsequently, at intervals of 1, 2, 4, 6, 8, and 12 weeks, 5 male Wistar rats in each group were chosen randomly for 24 h urinary protein quantitative measurements (24 h UPQM), and determination of plasma tumor necrosis factor alpha (TNF-alpha), angiopoietin-1 (Ang1), and angiopoietin-2 (Ang2) levels. Kidney sections were examined by electron microscopy, Periodic Acid Schiff (PAS) staining, immunohistochemical staining and in situ hybridization histochemistry. As glomerulosclerosis progressed in the DRB group, expression of Ang1 mRNA and protein in glomeruli decreased and expression of TNF-alpha protein, Ang2 mRNA and protein in glomeruli increased. Expression of Ang1 mRNA and protein in glomeruli were negatively correlated with 24 h UPQM, Fn protein expression, and mean area of extracellular matrix (MAECM). In comparison, expression of Ang2 mRNA and protein in glomeruli were positively correlated with 24 h UP...Continue Reading

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Citations

Sep 12, 2006·Journal of Huazhong University of Science and Technology. Medical Sciences = Hua Zhong Ke Ji Da Xue Xue Bao. Yi Xue Ying De Wen Ban = Huazhong Keji Daxue Xuebao. Yixue Yingdewen Ban·Yuanhang LuZhonghua Zhu
Oct 20, 2009·Current Opinion in Nephrology and Hypertension·Adrian S Woolf
Jun 1, 2008·Electrolyte & Blood Pressure : E & BP·Won Kim
Jul 13, 2007·Journal of the American Society of Nephrology : JASN·Belinda DavisLuigi Gnudi
Jul 13, 2007·Journal of the American Society of Nephrology : JASN·Mark P de Caestecker
Sep 19, 2008·Journal of the American Society of Nephrology : JASN·Adrian S WoolfDavid A Long
Nov 13, 2015·Clinical Science·Luigi GnudiDavid A Long
Feb 15, 2008·American Journal of Physiology. Renal Physiology·Valentina CâmpeanKerstin Amann
Jul 17, 2010·Biochemical and Biophysical Research Communications·Xia GaoXiliang Zha

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