Changes in astroglial GLT-1 expression after neural transplantation or stab wounds

Experimental Neurology
J M KrumJ M Rosenstein

Abstract

Uncontrolled release of glutamate from damaged brain initiates events that result in excitotoxic neuronal death. Glutamate uptake by specialized astroglial transporters is essential for control of extracellular glutamate levels. Many studies have demonstrated a reduction in astrocytic GLT-1 expression after different forms of injury. Because extensive neuronal death does not occur after direct cortical stab wounds and viable developing neurons populate fetal CNS grafts, we hypothesized that reactive astroglia associated with these procedures might maintain or up-regulate GLT-1. We examined the temporal and spatial distribution of GLT-1, GFAP and nestin proteins by confocal double-label immunohistochemistry combined with a new methodology in which precise brain areas are microdissected and analyzed for protein content by immunoaffinity chromatography. In stab wounds, GLT-1 protein content did not change compared to normal cortex, as determined by direct protein measurements; GLT-1 colocalized with nestin- and GFAP(+) astroglia adjacent to the lesion. In contrast, host reactive astroglia adjacent to grafts significantly upregulated GLT-1 by 3 days postoperative. The GFAP protein analysis suggests that increased GLT-1 is not the r...Continue Reading

References

Feb 1, 1986·Annals of Neurology·S M Rothman, J W Olney
May 15, 1988·The Journal of Comparative Neurology·J M Krum, J M Rosenstein
Mar 1, 1989·Experimental Neurology·J M Krum, J M Rosenstein
Jul 1, 1988·Journal of Chromatography·J V Babashak, T M Phillips
Jan 1, 1988·Experimental Brain Research·M FonsecaA Fairén
Jul 1, 1995·Annals of Neurology·J D RothsteinR W Kuncl
Oct 14, 1994·Brain Research. Developmental Brain Research·G D RosenA M Galaburda
Sep 1, 1994·Neuron·J D RothsteinR W Kuncl
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J D RothsteinR W Kuncl
Jun 1, 1993·Annals of Emergency Medicine·J T Povlishock
Oct 1, 1995·The European Journal of Neuroscience·L M LevyN C Danbolt
Nov 1, 1996·Journal of Chemical Neuroanatomy·O P OttersenR Torp
Sep 2, 1998·Cell and Tissue Research·C C Stichel, H W Müller
Oct 29, 1998·Journal of Chromatography. B, Biomedical Sciences and Applications·T M Phillips, J M Krum
Oct 13, 1999·Brain Research·S Sahin KayaM Chopp

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Citations

Jul 1, 2006·Journal of Neuroinflammation·Hsiao-Wen LinSteven W Levison
Jul 25, 2013·Journal of Molecular Endocrinology·Carla CarusoMercedes Lasaga
Nov 28, 2007·Journal of Neuroimmune Pharmacology : the Official Journal of the Society on NeuroImmune Pharmacology·Krishnan Sriram, James P O'Callaghan
Feb 21, 2004·The Journal of Pain : Official Journal of the American Pain Society·Alvin J BeitzLaura Eikmeier
Mar 19, 2016·Experimental Neurology·Hülya KıraySusan C Barnett
Oct 12, 2004·Experimental Neurology·Adrian G DervanGloria E Meredith
Nov 25, 2006·Journal of Neural Engineering·Andrew A SharpDiego Restrepo
Jul 11, 2006·Journal of Neuro-oncology·Ilaria Tamagno, Davide Schiffer

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