PMID: 8996693Jan 1, 1996Paper

Changes in binding affinity of a monoclonal antibody to a platelet binding domain of fibrinogen adsorbed to biomaterials

Journal of Biomaterials Science. Polymer Edition
John M GrunkemeierT A Horbett

Abstract

Previously, we found that when fibrinogen-coated polyurethanes resided in a buffer for a period of time (the 'residence time') platelet adhesion to these materials decreased. Other changes in adsorbed fibrinogen such as decreases in polyclonal antibody binding and SDS elutability supported the conclusion that fibrinogen undergoes postadsorptive conformational changes. Subsequently we measured the binding of monoclonal antibodies to the three putative platelet binding sites on fibrinogen, using a single mid-range concentration of antibody. We found that binding of a monoclonal antibody to the platelet binding site at the C-terminus of the gamma chain of fibrinogen changed little with residence time, while binding of monoclonal antibodies to the other two putative binding sites on fibrinogen either increased with residence time (RGDF at A alpha 95-98), or first increased and then decreased with residence time (RGDS at A alpha 572-575). In the current study, we measured antibody binding affinity, Ka, by measuring antibody binding at a series of antibody concentrations. This is a more sensitive method for detecting changes in adsorbed fibrinogen than measuring antibody binding from a single antibody concentration. The Ka was determ...Continue Reading

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Citations

Oct 27, 2006·Annals of Biomedical Engineering·Ayman HamedMichael Cho
Mar 25, 2009·Annals of Biomedical Engineering·Jiandi WanValentine I Vullev
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·N M LambaS L Cooper
Nov 7, 1998·Journal of Biomaterials Science. Polymer Edition·T A HorbettB D Ratner
Jan 1, 1997·Journal of Biomaterials Science. Polymer Edition·S M PeckhamS M Slack
Dec 5, 2002·Journal of Biomaterials Science. Polymer Edition·Rumiana TzonevaDieter Paul
Apr 28, 2006·Biopolymers·Lilia A ChtcheglovaAndré Haeberli
Nov 23, 2006·Journal of Biomedical Materials Research. Part a·T M MassaJ P Santerre
Jul 9, 1999·Journal of Biomedical Materials Research·V BalasubramanianS M Slack
Jul 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Pranav SomanChristopher A Siedlecki

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