Abstract
Previously, we found that when fibrinogen-coated polyurethanes resided in a buffer for a period of time (the 'residence time') platelet adhesion to these materials decreased. Other changes in adsorbed fibrinogen such as decreases in polyclonal antibody binding and SDS elutability supported the conclusion that fibrinogen undergoes postadsorptive conformational changes. Subsequently we measured the binding of monoclonal antibodies to the three putative platelet binding sites on fibrinogen, using a single mid-range concentration of antibody. We found that binding of a monoclonal antibody to the platelet binding site at the C-terminus of the gamma chain of fibrinogen changed little with residence time, while binding of monoclonal antibodies to the other two putative binding sites on fibrinogen either increased with residence time (RGDF at A alpha 95-98), or first increased and then decreased with residence time (RGDS at A alpha 572-575). In the current study, we measured antibody binding affinity, Ka, by measuring antibody binding at a series of antibody concentrations. This is a more sensitive method for detecting changes in adsorbed fibrinogen than measuring antibody binding from a single antibody concentration. The Ka was determ...Continue Reading
References
Sep 1, 1975·Journal of Biomedical Materials Research·B D RatnerS D Hauschka
Nov 10, 1992·Biochemistry·M BlumensteinJ Hawiger
Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D H FarrellE W Davie
Jun 1, 1992·Journal of Biomedical Materials Research·J A ChinnB D Ratner
Feb 1, 1992·Journal of Biomedical Materials Research·I A FeuersteinT A Horbett
May 18, 1990·Cell·W G LaverS J Smith-Gill
Dec 1, 1990·The Journal of Cell Biology·I F CharoD A Cheresh
May 1, 1991·The American Journal of Physiology·E ShibaE W Salzman
Apr 1, 1991·Journal of Biomedical Materials Research·J A ChinnB D Ratner
Sep 8, 1989·Cell·D A ChereshZ M Ruggeri
Nov 1, 1988·The Journal of Cell Biology·E A WaynerT J Kunicki
Dec 1, 1988·Proceedings of the Society for Experimental Biology and Medicine·M A Packham
Jul 1, 1986·Journal of Biomedical Materials Research·T A HorbettS R Hanson
Jul 16, 1982·Biochemical and Biophysical Research Communications·M KloczewiakJ Hawiger
Apr 10, 1984·Biochemistry·M KloczewiakJ Hawiger
Jun 15, 1993·The Biochemical Journal·S S Smyth, L V Parise
Jan 1, 1994·Journal of Biomaterials Science. Polymer Edition·T A Horbett, K R Lew
Sep 1, 1981·Journal of Biomedical Materials Research·T A Horbett
Citations
Oct 27, 2006·Annals of Biomedical Engineering·Ayman HamedMichael Cho
Mar 25, 2009·Annals of Biomedical Engineering·Jiandi WanValentine I Vullev
Jul 14, 2010·The Journal of Adhesion·Stéphanie Le ClairZhan Chen
Jul 22, 2009·Journal of Biomaterials Science. Polymer Edition·Wei-Bor TsaiHsiu-Wen Chien
Mar 27, 2001·Journal of Biomaterials Science. Polymer Edition·N M LambaS L Cooper
Apr 20, 2005·The Journal of Chemical Physics·Victoria Castells, Paul R Van Tassel
Jul 6, 2000·Journal of Biomedical Materials Research·J M GrunkemeierT A Horbett
Feb 9, 2002·Journal of Biomedical Materials Research·A R JahangirJ P Santerre
Nov 19, 2003·Journal of Biomedical Materials Research. Part a·Wei-Bor TsaiThomas A Horbett
Aug 21, 2004·Journal of Biomaterials Science. Polymer Edition·W B TsaiT A Horbett
Nov 7, 1998·Journal of Biomaterials Science. Polymer Edition·T A HorbettB D Ratner
Jan 1, 1997·Journal of Biomaterials Science. Polymer Edition·S M PeckhamS M Slack
Dec 5, 2002·Journal of Biomaterials Science. Polymer Edition·Rumiana TzonevaDieter Paul
Apr 28, 2006·Biopolymers·Lilia A ChtcheglovaAndré Haeberli
Nov 23, 2006·Journal of Biomedical Materials Research. Part a·T M MassaJ P Santerre
Jul 23, 2005·Journal of Biomedical Materials Research. Part a·Yuguang WuThomas A Horbett
Jan 4, 2012·Journal of Biomedical Materials Research. Part a·Vitaliy OvodDonald L Elbert
Dec 10, 2003·Journal of Biomaterials Science. Polymer Edition·Meng ChenShigemasa Osaki
Jul 9, 1999·Journal of Biomedical Materials Research·V BalasubramanianS M Slack
Jul 12, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Pranav SomanChristopher A Siedlecki