Changes in bioactive components of Aristolochia tagala .Cham, a rare species of medicinal importance during its in vitro development through direct regeneration

BioRxiv : the Preprint Server for Biology
Remya MohanrajMUTHARAIAN VN

Abstract

Tissue culture propagation system was developed for Aristolochia tagala, a threatened medicinal plant, using apical bud explants. The most effective medium was found to be MS medium supplemented with BAP (3 μM), KIN (0.5 μM) and activated charcoal (0.1%). The addition of activated charcoal helped in circumventing the problem of polyphenol exudation from the explants which hampered the regeneration of adventitious shoots. A maximum of 12.6 shoots were obtained on average from the apical bud explants after 25 days of inoculation. Well developed shoots were rooted on MS medium supplemented with indole acetic acid (1.5 μM), Kinetin (1.5 μ M) and 6-benzylaminopurine (0.5 μM). Regenerated shoots from the apical buds were successfully rooted and acclimated to greenhouse conditions. Qualitative and quantitative analysis of bioactive compounds was done at various stages of development, so as establish the effect of culture conditions on the production of bioactive components. Comparisons were made between three types of plant material from the same clone: leaves from field-grown plant, leaves from in vitro apical bud cultures and leaf derived callus. It was observed that the leaf derived callus showed the presence of components which we...Continue Reading

Related Concepts

Activated Charcoal
Medical Devices
Plants, Medicinal
Natural Regeneration
Mass Spectrometry
Tissue Culture
N6-furfuryladenine
Polyphenols
Apical
Plant Leaves

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