PMID: 9539308Apr 16, 1998Paper

Changes in carbohydrate metabolism of testicular germ cells during meiosis in the rat

European Journal of Endocrinology
M BajpaiB S Setty

Abstract

A study was undertaken to estimate the activities of the key enzymes of glycolysis, the pentose phosphate pathway and the tricarboxylic acid (TCA) cycle in purified rat spermatocytes and spermatids, which have been shown to die in glucose-containing medium and require lactate/pyruvate for maintaining normal ATP concentrations. The aim was to elucidate the changes in the glycolytic and oxidative potential of germ cells undergoing meiosis. Pachytene spermatocytes and round spermatids from adult rat testis were purified to approximately 90% purity by trypsin digestion followed by a combination of centrifugal elutriation and Percoll density gradient centrifugation. After the purity and viability of these cells had been established, their contents of hexokinase, phosphofructokinase, lactate dehydrogenase (LDH) and LDH-X of glycolysis, glucose 6-phosphate dehydrogenase of the pentose phosphate pathway and citrate synthase, aconitase, malate dehydrogenase and 2-oxoglutarate dehydrogenase of the TCA cycle were estimated. These enzymes were also estimated in epididymal spermatozoa for comparison with the testicular germ cells. The results indicate greater activity of glycolytic and pentose phosphate pathway enzymes in spermatocytes than...Continue Reading

Citations

Aug 26, 2006·Bulletin of Experimental Biology and Medicine·O N Shevantaeva, Yu I Kosyuga
Apr 3, 2004·Free Radical Biology & Medicine·R John AitkenEileen A McLaughlin
Aug 26, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·E Ruiz-PesiniM J López-Pérez
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Feb 13, 2016·Frontiers in Endocrinology·Shabana AnjumKazuyoshi Tsutsui
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