Changes in carotenoid content and biosynthetic gene expression in juice sacs of four orange varieties (Citrus sinensis) differing in flesh fruit color

Journal of Agricultural and Food Chemistry
Anne-Laure FanciullinoRaphael Morillon

Abstract

The contribution of carotenoid composition to the color range of the fruit juice sacs of four orange varieties (Citrus sinensis) differing in flesh color, namely, Shamouti (normal orange color), Sanguinelli ("blood cultivar" purple color), Cara Cara navel (pink-reddish), and Huang pi Chen (yellowish color), was investigated. To this end, qualitative and quantitative analyses of carotenoid contents were first performed by high-performance liquid chromatography (HPLC) using a C30 column and a photodiode array detector in February, at a late developmental fruit stage. Concomitantly, transcript levels of Dxs, the gene controlling the first step of the MEP pathway, and six genes involved in beta,beta-xanthophyll biosynthesis (Psy, Pds, Zds, Lcy-b, Hy-b, and Zep) were determined in August, November, and February. Transcript level measurement was carried out by real-time RT-PCR on total RNA from juice sacs. The four orange varieties displayed different carotenoid profiles. Shamouti and Sanguinelli oranges accumulated mainly beta,beta-xanthophylls as expected in typically colored oranges, whereas Cara Cara navel orange accumulated linear carotenes in addition to cis-violaxanthin. Huang pi Chen fruit flesh orange was characterized by a ...Continue Reading

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Citations

Jul 4, 2009·Journal of Experimental Botany·Charles Ampomah-DwamenaAndrew C Allan
Feb 9, 2012·Journal of the Science of Food and Agriculture·Florine Poiroux-GonordLaurent Urban
Jan 22, 2013·Physiologia Plantarum·Florine Poiroux-GonordLaurent Urban
Aug 14, 2012·Physiologia Plantarum·Florine Poiroux-GonordLaurent Urban
May 23, 2015·Food & Function·María Jesús RodrigoLorenzo Zacarías
Jun 6, 2013·Oxidative Medicine and Cellular Longevity·Giuseppe GrossoAlessandro Scuderi
Sep 16, 2014·Plant Science : an International Journal of Experimental Plant Biology·Xu WeiFrederick G Gmitter
Feb 2, 2017·Journal of Agricultural and Food Chemistry·Elisa BrasiliFranco M Lajolo
Jul 31, 2020·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Jaime Zacarías-GarcíaLorenzo Zacarías
Jan 30, 2021·Plant Communications·Muhammad Junaid RaoQiang Xu
Mar 18, 2010·Journal of Agricultural and Food Chemistry·Mery Dafny-YalinRachel Amir
Jun 22, 2010·Archives of Biochemistry and Biophysics·Manuel Rodríguez-Concepción

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