Changes in Caspase-3, B Cell Leukemia/Lymphoma-2, Interleukin-6, Tumor Necrosis Factor-α and Vascular Endothelial Growth Factor Gene Expression after Human Umbilical Cord Blood Derived Mesenchymal Stem Cells Transfusion in Pulmonary Hypertension Rat Models

Korean Circulation Journal
Kwan Chang KimYoung Mi Hong

Abstract

Failure of vascular smooth muscle apoptosis and inflammatory response in pulmonary arterial hypertension (PAH) is a current research focus. The goals of this study were to determine changes in select gene expressions in monocrotaline (MCT)-induced PAH rat models after human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) transfusion. The rats were separated into 3 groups i.e., control group (C group), M group (MCT 60 mg/kg), and U group (hUCB-MSCs transfusion) a week after MCT injection. TUNEL assay showed that the U group had significantly lowered positive apoptotic cells in the lung tissues, as compared with the M group. mRNA of caspase-3, B cell leukemia/lymphoma (Bcl)-2, interleukin (IL)-6, tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF) in the lung tissues were greatly reduced at week 4 in the U group. Immunohistochemical staining of the lung tissues also demonstrated a similar pattern, with the exception of IL-6. The protein expression of caspase-3, Bcl-2 VEGF, IL-6, TNF-α and brain natriuretic peptide in the heart tissues were significantly lower in the U group, as compared with the M group at week 2. Furthermore, the protein expression of VEGF, IL-6 and BNP in the heart tissue...Continue Reading

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Citations

Mar 10, 2016·Pediatric Research·Hyeryon LeeYoung Mi Hong
Mar 5, 2017·International Journal of Cardiology·Enze JinSung-Whan Kim
Nov 8, 2018·Korean Circulation Journal·Kyung Lim Yoon
Mar 8, 2019·Stem Cell Research & Therapy·Colin M SuenManoj M Lalu
Sep 26, 2020·Frontiers in Aging Neuroscience·Poornima VenkatJieli Chen
Sep 18, 2021·Frontiers in Physiology·Lingjie LuoDeming Gou

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Methods Mentioned

BETA
light microscopy
PCR
protein assay
electrophoresis

Software Mentioned

analySIS
SPSS
Primer Express

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