Changes in Cell Composition of Umbilical Cord Blood and Functional Activity of Hematopoietic Stem Cells during Cryogenic Storage and Repeated Freezing/Thawing Cycles

Bulletin of Experimental Biology and Medicine
Yu A RomanovG T Sukhikh

Abstract

We analyzed changes in cell composition of umbilical cord blood and functional activity of hematopoietic stem cells during cryogenic storage and after repeated freezing/thawing cycles. It was found that repeated freezing/thawing cycles performed according to the optimal programmable freezing protocol did not significantly affect viability and functional activity of hematopoietic stem cells. When fast freezing program was used, the cells completely lost their capacity to form colonies in semisolid medium, despite high viability parameters in the test with 7-AAD.

References

Jan 30, 2010·Seminars in Hematology·Hal E Broxmeyer
May 16, 2013·Blood·Karen K BallenHal E Broxmeyer
Sep 26, 2013·Frontiers in Oncology·Leland MethenyMarcos de Lima
Mar 25, 2015·Cytotherapy·Jessica M Sun, Joanne Kurtzberg
Jul 3, 2015·Stem Cell Research & Therapy·Santiago RouraAntoni Bayes-Genis

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Citations

Feb 28, 2017·Bulletin of Experimental Biology and Medicine·Yu A RomanovG T Sukhikh

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