Changes in cell wall polysaccharide composition, gene transcription and alternative splicing in germinating barley embryos

Journal of Plant Physiology
Qisen ZhangChengdao Li

Abstract

Barley (Hordeum vulgare L.) seed germination initiates many important biological processes such as DNA, membrane and mitochondrial repairs. However, little is known on cell wall modifications in germinating embryos. We have investigated cell wall polysaccharide composition change, gene transcription and alternative splicing events in four barley varieties at 24h and 48 h germination. Cell wall components in germinating barley embryos changed rapidly, with increases in cellulose and (1,3)(1,4)-β-D-glucan (20-100%) within 24h, but decreases in heteroxylan and arabinan (3-50%). There were also significant changes in the levels of type I arabinogalactans and heteromannans. Alternative splicing played very important roles in cell wall modifications. At least 22 cell wall transcripts were detected to undergo either alternative 3' splicing, alternative 5' splicing or intron retention type of alternative splicing. These genes coded enzymes catalyzing synthesis and degradation of cellulose, heteroxylan, (1,3)(1,4)-β-D-glucan and other cell wall polymers. Furthermore, transcriptional regulation also played very important roles in cell wall modifications. Transcript levels of primary wall cellulase synthase, heteroxylan synthesizing and n...Continue Reading

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Citations

Apr 1, 2016·PloS One·Qisen ZhangChengdao Li
Mar 27, 2021·Scientific Data·Linda MilneCraig G Simpson
Aug 10, 2021·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Tariq PervaizLingfei Shangguan

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