DOI: 10.1101/489195Dec 6, 2018Paper

Changes in clock gene expression in esophagus in rat reflux esophagitis

BioRxiv : the Preprint Server for Biology
Atsushi HashimotoYasuhiro Fujiwara


Background and Aims: Gastroesophageal reflux disease (GERD) is strongly associated with sleep disturbances. Clock genes harmonize circadian rhythms by their periodic expression and regulate several physiological functions. However, the association between clock genes and GERD is still unknown. We investigated whether reflux esophagitis affects circadian variability of clock genes in the esophagus and other organs using a rat reflux esophagitis model. Methods: Reflux esophagitis was induced in 7-week-old male Wistar rats. Sham-operated rats were used as controls. Rats were sacrificed at 09:00 (light period) and 21:00 (dark period) 3 days (acute phase) and 21 days (chronic phase) after induction of esophagitis. The expression levels of clock gene mRNAs such as Per1, Per2, Per3, Cry1, Cry2, Arntl, and Clock in the esophagus were investigated by qPCR. Arntl expression was examined in stomach, small intestine, colon, and liver tissues. Serum melatonin and IL-6 levels were measured by ELISA. Results: Histological examination of reflux esophagitis mainly revealed epithelial defects with marked inflammatory cell infiltration in the acute phase, and mucosal thickening with basal cell hyperplasia in the chronic phase. Circadian variabili...Continue Reading

Related Concepts

Circadian Rhythms
Colon Structure (Body Structure)
Enzyme-Linked Immunosorbent Assay
Epithelial Cells
Gastroesophageal Reflux Disease
Intestines, Small

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