Changes in Clostridium (Clostridioides) difficile PCR-Ribotype Distribution and Antimicrobial Resistance in a German Tertiary Care Hospital Over the Last 10 Years

Current Microbiology
E PiepenbrockN Jazmati

Abstract

Clostridium (Clostridioides) difficile ribotype 027 (RT027) was detected in Germany for the first time in 2007 during an outbreak in the region of Trier, Rhineland-Palatinate and is today the most prevalent ribotype (RT) in Europe. We aimed to determine the changes in RT distribution and corresponding antimicrobial resistance in clinical C. difficile isolates between two time points (2007 and 2017) in one tertiary care hospital in Germany. C. difficile isolates recovered in 2007 and in 2017 (80 isolates per year, respectively) from patients at a Tertiary Care University Hospital in North-Rhine Westphalia were analyzed. Isolates were characterized by ribotyping and susceptibility testing using gradient tests (metronidazole, vancomycin) and the disk diffusion method (moxifloxacin). Between 2007 and 2017, a clear switch from RT001 [18.75% (n = 15) in 2007 versus 3.75% (n = 3) in 2017 P = 0.003] to RT027 [0% in 2007 versus 21.25% (n = 17) in 2017] was evident. While minimal inhibitory concentrations (MICs) of vancomycin were stable, a significant metronidazole MIC creep was determined (MIC50 = 0.047 in 2007 versus MIC50 = 0.094 in 2017, P < 0.0001 using the Man-Whitney test). We detected one metronidazole-resistant isolate (0.6%). ...Continue Reading

References

Jul 14, 2001·Antimicrobial Agents and Chemotherapy·G AckermannS H Cohen
Oct 27, 2001·The Journal of Antimicrobial Chemotherapy·J S BrazierM H Wilcox
Sep 14, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·F BarbutUNKNOWN European Study Group on Clostridium difficile (ESGCD)
Aug 12, 2008·The Journal of Antimicrobial Chemotherapy·Simon D BainesMark H Wilcox
Sep 24, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Abraham GoorhuisEd J Kuijper
Nov 26, 2009·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M J T CrobachE J Kuijper
Mar 31, 2010·Emerging Infectious Diseases·Nils Henning ZaissUlrich Nübel
Sep 15, 2010·Zeitschrift für Gastroenterologie·Andreas JansenT Eckmanns
Nov 19, 2010·Lancet·Martijn P BauerUNKNOWN ECDIS Study Group
Dec 14, 2011·Epidemiology and Infection·M ReilS Borgmann
Apr 28, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M P M HensgensUNKNOWN European Society of Clinical Microbiology and Infectious Diseases Study Group for Clostridium difficile (ESGCD)
Jun 8, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L T ErikstrupU S Justesen
Oct 30, 2012·The Journal of Antimicrobial Chemotherapy·Ines MouraPaola Mastrantonio
Nov 2, 2012·Bundesgesundheitsblatt, Gesundheitsforschung, Gesundheitsschutz·L von MüllerM Herrmann
Jan 26, 2013·PloS One·Tarah LynchUNKNOWN Canadian Nosocomial Infection Surveillance Program (CNISP)
Feb 24, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J FreemanUNKNOWN Pan-European Longitudinal Surveillance of Antibiotic Resistance among Prevalent Clostridium difficile Ribotypes' Study Group
Apr 12, 2016·Anaerobe·Mardjan Arvand, Gudrun Bettge-Weller
Jun 14, 2016·Anaerobe·Bo-Yang TsaiPei-Jane Tsai
Jul 30, 2016·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·Kerrie A DaviesUNKNOWN EUCLID study group
Aug 24, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M J T CrobachM C Vos
Oct 27, 2017·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·J FreemanUNKNOWN Pan-European Longitudinal Surveillance of Antibiotic Resistance among Prevalent Clostridium difficile Ribotypes Study Group
May 4, 2018·Journal of Clinical Microbiology·N JazmatiH Seifert

❮ Previous
Next ❯

Citations

Feb 1, 2020·Nature Communications·Ilse M BoekhoudWiep Klaas Smits
Sep 27, 2020·Antimicrobial Resistance and Infection Control·Mohammad SholehEbrahim Kouhsari
Apr 21, 2021·The Journal of Antimicrobial Chemotherapy·Ilse M BoekhoudUNKNOWN COMBACTE-CDI Consortium
Apr 30, 2021·International Journal of Medical Microbiology : IJMM·Ahmed Mohamed Mostafa AbdrabouFabian K Berger
Aug 10, 2021·Infection Prevention in Practice·Vanda Marujo, Mardjan Arvand

❮ Previous
Next ❯

Methods Mentioned

BETA
ribotyping
PCR
ELISA

Software Mentioned

GeneXpert

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.