Changes in collagen cross-linking in bleomycin-induced pulmonary fibrosis.

Journal of Biochemical Toxicology
K M ReiserH R Witschi

Abstract

Collagen cross-linking was analyzed in lungs of rats, two, four, and ten weeks after intratracheal instillation of 1.5 units of bleomycin. Similar analyses were performed on lungs of mice 18 months after intratracheal instillation of bleomycin with or without subsequent exposure to 70% oxygen (O2) for 72 hours. Lungs were analyzed to determine the content of the reduced difunctional cross-links dihydroxylysinonorleucine (DHLNL) and hydroxylysinonorleucine (HLNL) and of the nonreducible trifunctional cross-link hydroxypyridinium (OHP). Ratios of DHLNL:HLNL were elevated in the rat lungs at two and four weeks, due to increased levels of DHLNL. There were no changes in the difunctional cross-links in any of the mouse lungs. Hydroxypyridinium content was elevated in the rat lungs at ten weeks and in the mouse lungs exposed to bleomycin and oxygen. We conclude that increases in DHLNL may serve as an early indicator that potentially "fibrotic collagen" is being synthesized in lungs acutely exposed to fibrogenic stimuli, while increases in OHP may serve as a permanent marker of a fibrogenic event that could have occurred months to years earlier.

References

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Citations

Jan 1, 1990·The International Journal of Biochemistry·J A LastK M Reiser
Oct 17, 1995·Biochimica Et Biophysica Acta·L C Armstrong, J A Last
Jul 26, 1991·Biochimica Et Biophysica Acta·K M ReiserJ R Williamson
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Aug 1, 1993·The American Review of Respiratory Disease·J A LastH Witschi
Sep 17, 2011·American Journal of Respiratory and Critical Care Medicine·Martin R J Kolb, Jack Gauldie
Jun 1, 1990·American Journal of Respiratory Cell and Molecular Biology·J A LastK M Reiser
Mar 7, 2001·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·A EspañaE Quintanilla
Jun 6, 2000·Toxicology·B E Van HoozenJ A Last
Jan 7, 1998·Biochemical Pharmacology·S N GiriM D Pierschbacher
Jan 15, 2013·Critical Reviews in Oncology/hematology·Marios FroudarakisEvangelos Briasoulis
Aug 9, 2005·Pathologie-biologie·Kornelia Molnarne SzauterKatalin Csiszar
Jul 1, 1987·Toxicology and Applied Pharmacology·K M ReiserJ A Last
Nov 13, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Eleni G TzortzakiDonald R Gerecke
Jun 14, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Camille BrasseletAntoine Lafont
Apr 9, 2013·American Journal of Physiology. Lung Cellular and Molecular Physiology·William McKleroyKamran Atabai
Dec 12, 2018·Journal of Cellular and Molecular Medicine·Heidi SchilterWolfgang Jarolimek
Dec 2, 2020·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Chelsey L DunhamSpencer P Lake

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