Changes in composition of caecal microbiota associated with increased colon inflammation in interleukin-10 gene-deficient mice inoculated with Enterococcus species

Nutrients
Shalome A BassettNicole C Roy

Abstract

Human inflammatory bowel disease (IBD) is a chronic intestinal disease where the resident microbiota contributes to disease development, yet the specific mechanisms remain unclear. Interleukin-10 gene-deficient (Il10-/-) mice develop inflammation similar to IBD, due in part to an inappropriate response to commensal bacteria. We have previously reported changes in intestinal morphology and colonic gene expression in Il10-/- mice in response to oral bacterial inoculation. In this study, we aimed to identify specific changes in the caecal microbiota associated with colonic inflammation in these mice. The microbiota was evaluated using pyrotag sequencing, denaturing gradient gel electrophoresis (DGGE) and quantitative real-time PCR. Microbiota profiles were influenced by genotype of the mice and by bacterial inoculation, and a strong correlation was observed between the microbiota and colonic inflammation scores. Although un-inoculated Il10-/- and C57 mice had similar microbiota communities, bacterial inoculation resulted in different changes to the microbiota in Il10-/- and C57 mice. Inoculated Il10-/- mice had significantly less total bacteria than un-inoculated Il10-/- mice, with a strong negative correlation between total bacte...Continue Reading

References

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Citations

May 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Stefan O ReberChristopher A Lowry
Oct 25, 2016·International Journal of Molecular Sciences·Lynnette R Ferguson, Matthew P G Barnett
May 17, 2019·Pflügers Archiv : European journal of physiology·Ervice Pouokam, Martin Diener
Apr 30, 2017·Microbiome·Mikayla A BortonKelly C Wrighton
Sep 28, 2018·Frontiers in Immunology·Valentina P SebastiánSusan M Bueno
Jan 21, 2016·Molecular Ecology·O Paliy, V Shankar
Sep 23, 2020·Cellular and Molecular Gastroenterology and Hepatology·David C MontroseAndrew J Dannenberg
Dec 18, 2020·Frontiers in Physiology·Qiulan LvShichao Xing

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Methods Mentioned

BETA
PCR
Electrophoresis
PCA

Software Mentioned

R
vegan
Greengenes
UniFrac
agricolae package
UCLUST
BioNumerics
USEARCH
Qiime

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