PMID: 2120197Oct 1, 1990Paper

Changes in composition of peptidoglycan during maturation of the cell wall in pneumococci

Journal of Bacteriology
H Laitinen, A Tomasz

Abstract

An experimental system which allows the selective reisolation and structural analysis of a newly made (nascent) segment of pneumococcal peptidoglycan at various times after its incorporation into the preexisting old cell wall was developed. Age-related changes were observed in each one of the major nine wall peptide components resolvable by a high-performance liquid chromatography method. The nascent wall segment (made in 1.7% of a generation time) contained 60% of its peptides as the alanyl-isoglutamyl-lysine tripeptide monomer, 12% as the directly cross-linked peptide dimer (tri-tetra peptide), and a total of 2% as the two major peptide trimers. In the mature wall segment reisolated 1 h later (1 generation time), the proportion of the tripeptide monomer dropped to 40%, while the major dimer and trimers increased to 23% and 8%, respectively. The age-related structural changes were completely inhibited by cefotaxime. The observations indicate that covalent bonds in the structure of pneumococcal peptidoglycan undergo substantial secondary rearrangements after incorporation into the preexisting wall. These changes are likely to be related to the movement of the conserved cell wall segments within the cell surface during cell divi...Continue Reading

References

Jan 1, 1975·Journal of Supramolecular Structure·A TomaszP Fletcher
Jan 1, 1985·Molecular & General Genetics : MGG·E GarcíaR López
May 1, 1988·Journal of Bacteriology·J F Garcia-BustosA Tomasz
Jul 1, 1985·Journal of Bacteriology·E W Goodell
Aug 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·A TomaszR Ziegler
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·L G Burman, J T Park
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M A de Pedro, U Schwarz

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Citations

Jan 16, 2008·FEMS Microbiology Reviews·Waldemar VollmerMiguel A de Pedro
Jul 16, 2013·Environmental Microbiology·Orietta MassiddaWaldemar Vollmer
Oct 14, 2017·FEMS Microbiology Reviews·Ditte Høyer EngholmRikke Schmidt Kjærgaard
Dec 24, 2011·Analytical Biochemistry·Nhat Khai BuiWaldemar Vollmer
Jun 8, 2019·Microbiology Spectrum·Waldemar VollmerAlexander Tomasz

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