Changes in distribution of basic nuclear proteins and chromatin organization during spermiogenesis in the greater bandicoot rat, Bandicota indica

Cell and Tissue Research
Pakawadee WorawittayawongPrapee Sretarugsa

Abstract

Male germ cells of the greater bandicoot rat, Bandicota indica, have recently been categorized into 12 spermiogenic steps based upon the morphological appearance of the acrosome and nucleus and the cell shape. In the present study, we have found that, in the Golgi and cap phases, round spermatid nuclei contain 10-nm to 30-nm chromatin fibers, and that the acrosomal granule forms a huge cap over the anterior pole of nucleus. In the acrosomal phase, many chromatin fibers are approximately 50 nm thick; these then thickened to 70-nm fibers and eventually became 90-nm chromatin cords that are tightly packed together into highly condensed chromatin, except where nuclear vacuoles occur. Immunocytochemistry and immunogold localization with anti-histones, anti-transition protein2, and anti-protamine antibodies suggest that histones remain throughout spermiogenesis, that transition proteins are present from step 7 spermatids and remain until the end of spermiogenesis, and that protamines appear at step 8. Spermatozoa from the cauda epididymidis have been analyzed by acid urea Triton X-100 polyacrylamide gel electrophoresis for basic nuclear proteins. The histones, H2A, H3, H2B, and H4, transitional protein2, and protamine are all present...Continue Reading

References

Jun 1, 1979·Journal of Ultrastructure Research·M Loir, J L Courtens
Nov 1, 1977·Experimental Cell Research·S R GrimesL S Hnilica
Jan 1, 1991·Progress in Nucleic Acid Research and Molecular Biology·R Oliva, G H Dixon
Mar 1, 1973·The American Journal of Anatomy·G B Dooher, D Bennett
Mar 1, 1981·Journal of Ultrastructure Research·J L Courtens, M Loir
May 1, 1982·The Journal of Cell Biology·R Balhorn
Apr 1, 1981·The American Journal of Anatomy·M Lalli, Y Clermont
Jun 1, 1995·Journal of Molecular Evolution·R QueraltG H Dixon
Sep 1, 1993·Journal of Reproduction and Fertility·W G Breed
May 20, 1998·Biochimie·D Wouters-TyrouP Sautière
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·Y E YuM L Meistrich
Mar 10, 2001·Molecular Reproduction and Development·N CaronG Boissonneault
Mar 14, 2002·Molecular Reproduction and Development·Michele CorzettRod Balhorn
Jul 26, 2002·The Journal of Biological Chemistry·Laurence BrewerRod Balhorn
May 14, 2003·Chromosoma·Marvin L MeistrichMing Zhao
May 31, 2003·The Journal of Biological Chemistry·Susan M Wykes, Stephen A Krawetz
Jul 9, 2004·Cytogenetic and Genome Research·D ChurikovA O Zalensky
Dec 1, 2004·Molecular Reproduction and Development·Sirikul ManochantrPrasert Sobhon
Apr 1, 2005·Nature·Sarah Kimmins, Paolo Sassone-Corsi

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