Changes in DNA methylation during somatic embryogenesis in Cucurbita pepo L

Plant Cell Reports
Dunja Leljak-LevanićSibila Jelaska

Abstract

Three pumpkin embryogenic lines were initiated on wounded zygotic embryos cultured on medium with or without 2,4-dichlorophenoxyacetic acid (2,4-D). Somatic embryo development was controlled by the availability of various compounds in the medium: presence/absence of 2,4-D, nitrogen sources. The highest rate of DNA methylation was in the early embryo stages, predominantly on MSC medium with 2,4-D and on auxin-free medium supplemented with 1.0 m M NH(4)Cl. DNA methylation was correlated with early embryo development in a manner that was not exclusively dependent on the presence/absence of exogenous auxin. DNA methylation decreased during embryo maturation on auxin-free MSC medium and on auxin-free MSC supplemented with 12.3 micro M 5-azacytidine (5-azaC). The embryogenic features of the pumpkin tissue were preserved, even after a 2-month treatment with 5-azaC.

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Citations

Apr 6, 2012·Plant Cell Reports·Yuepeng SongDeqiang Zhang
Sep 19, 2009·Journal of Chemical Biology·Omid KaramiAghil Mahmoudi Pour
Sep 26, 2015·Plant Cell Reports·Dunja Leljak-LevanićSnježana Mihaljević
Aug 3, 2011·Journal of Plant Physiology·Snježana MihaljevićSibila Jelaska
Aug 28, 2010·Journal of Applied Microbiology·D Leljak-LevanićM Curković-Perica
Sep 9, 2015·Frontiers in Plant Science·Clelia De-la-PeñaVíctor M Loyola-Vargas
Aug 11, 2016·Journal of Biological Research·Renata OrłowskaPiotr Tomasz Bednarek
Aug 26, 2009·Molecular Biology Reports·Omid Karami, Abbas Saidi
Oct 3, 2018·Frontiers in Plant Science·Barbara WójcikowskaMałgorzata D Gaj
Oct 20, 2018·International Journal of Molecular Sciences·Pedro Osorio-MontalvoClelia De-la-Peña
May 15, 2020·International Journal of Molecular Sciences·Teresa Hazubska-PrzybyłEmilia Pers-Kamczyc
Apr 13, 2021·Frontiers in Plant Science·Ming ZhangLingyun Zhang
Jul 6, 2014·BMC Genomics·Qingfen LiJunhui Wang

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