PMID: 8977528Dec 1, 1996Paper

Changes in eosinophil and leukocyte infiltration and expression of IL-6 and IL-7 messenger RNA in mite allergen patch test reactions in atopic dermatitis

The Journal of Allergy and Clinical Immunology
N YamadaK Tamaki

Abstract

Patch testing with crude dust mite extracts after removal of homy layers was performed on normal-appearing skin of 11 adult patients with atopic dermatitis and high mite-specific IgE antibody scores. Positive skin reactions were observed in 9 subjects. Skin biopsy specimens were obtained from positive reaction sites at 2, 6, 12, 24, and 48 hours after allergen challenge and subjected to histologic studies and extraction of messenger RNA (mRNA). Perivascular infiltration of small mononuclear cells began at 2 hours and was followed by eosinophilic infiltration at 6 hours, and the number of eosinophils continued to increase at 24 and 48 hours. In addition to the increased expression of IL-4, IL-5, and tumor necrosis factor-alpha mRNA during the time course detected by reverse-transcription polymerase chain reaction, mRNA of IL-6 and IL-7 was also up-regulated. After the removal of test patches with mite allergen, the number of eosinophils started to decrease in a time-dependent manner. Histopathologic findings at 48 hours after removal showed lymphocyte-dominant infiltration intermingled with occasional eosinophils. These mite allergen patch test reactions may provide a useful model for studying the pathogenesis of atopic eczema, ...Continue Reading

References

Feb 1, 1991·European Journal of Immunology·J SamaridisR Palacios
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Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·G D ChazenE M Shevach
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Dec 1, 1995·The Journal of Allergy and Clinical Immunology·N YamadaH Nakagawa

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Citations

Jun 5, 2003·Clinics in Dermatology·Vincent S Beltrani
Mar 20, 2012·Seminars in Immunology·Renata I MazzucchelliScott K Durum
Mar 27, 1998·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·W J FokkensE Eichhorn
Oct 5, 2017·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·K MalikE Guttman-Yassky

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