Changes in epidermal growth factor receptor expression in human bladder cancer cell lines following interferon-alpha treatment

The Journal of Urology
Xian-Jun QuD Goldstein

Abstract

We examined the regulation of epidermal growth factor (EGF) receptor (EGFR) expression in human bladder cancer cell lines by interferon-alpha (IFN-alpha), the ability of IFN-alpha to inhibit cell proliferation and the sensitivity of IFN-alpha pretreated cells to EGF. Cell proliferation was determined using crystal violet colorimetric and clonogenic assays. EGFR expression was measured by flow cytometry using specific antibody or ligand binding approaches. After IFN-alpha (100 IU/ml) treatment cell surface EGFR expression was upregulated in 6 of 11 and down-regulated in 2 of 11 bladder cancer cell lines. The over expression of cell surface EGFR peaked within 48 to 96 hours and increased by 35% to 241% in individual cell lines. High level cell surface EGFR correlated with intracellular EGFR expression. Cell growth inhibition by IFN-alpha coexisted with EGFR over expression in the 6 lines. IFN-alpha treated cells remained sensitive to EGF treatment. IFN-alpha transiently up-regulates EGFR expression and inhibits in vitro growth in some human bladder cancer cells. IFN-alpha does not prevent EGFR from binding EGF or signal transduction via the EGF-EGFR pathway. This may have clinical implications for improving treatment based on EGF...Continue Reading

References

Mar 1, 1994·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M CaragliaP Tagliaferri
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Apr 4, 2003·International Journal of Cancer. Journal International Du Cancer·Helmout ModjtahediSuzanne Eccles

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Citations

Jun 12, 2009·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Arlhee DiazEnrique Montero
Jun 18, 2009·Journal of Cancer Research and Clinical Oncology·Beata ZahorowskaJia-Lin Yang
Nov 11, 2014·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Lu WangXianjun Qu
Jan 24, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Francesca BruzzeseAlfredo Budillon

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