Changes in expression of the long non-coding RNA FMR4 associate with altered gene expression during differentiation of human neural precursor cells

Frontiers in Genetics
Veronica J PeschanskyClaes Wahlestedt

Abstract

CGG repeat expansions in the Fragile X mental retardation 1 (FMR1) gene are responsible for a family of associated disorders characterized by either intellectual disability and autism Fragile X Syndrome (FXS), or adult-onset neurodegeneration Fragile X-associated Tremor/Ataxia Syndrome. However, the FMR1 locus is complex and encodes several long non-coding RNAs, whose expression is altered by repeat expansion mutations. The role of these lncRNAs is thus far unknown; therefore we investigated the functionality of FMR4, which we previously identified. "Full"-length expansions of the FMR1 triplet repeat cause silencing of both FMR1 and FMR4, thus we are interested in potential loss-of-function that may add to phenotypic manifestation of FXS. Since the two transcripts do not exhibit cis-regulation of one another, we examined the potential for FMR4 to regulate target genes at distal genomic loci using gene expression microarrays. We identified FMR4-responsive genes, including the methyl-CpG-binding domain protein 4 (MBD4). Furthermore, we found that in differentiating human neural precursor cells, FMR4 expression is developmentally regulated in opposition to expression of both FMR1 (which is expected to share a bidirectional promote...Continue Reading

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Citations

Jan 26, 2016·International Journal of Molecular Sciences·Kun YanAirong Qian
Dec 8, 2015·Molecular and Cellular Neurosciences·Anita Bhattacharyya, Xinyu Zhao
Jun 22, 2017·CNS Neuroscience & Therapeutics·Jie TangWei Yang
Feb 15, 2020·Neuropathology and Applied Neurobiology·R D SalinasH Song
Mar 5, 2016·Reviews in the Neurosciences·Lei WangYing Xia
Dec 14, 2018·Frontiers in Neuroscience·Robert J Oliver, Chitra D Mandyam
Mar 6, 2021·Trends in Cell Biology·Fatemeh Mirzadeh AzadSalvatore Oliviero
Aug 6, 2021·Frontiers in Molecular Neuroscience·Rafaela PolicarpoConstantin d'Ydewalle
Aug 28, 2021·International Journal of Molecular Sciences·Luis M ValorRosario Marín

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Datasets Mentioned

BETA
GSE70817

Methods Mentioned

BETA
transfection
transfections
PCR
immunoprecipitation

Software Mentioned

Affymetrix GeneChip Command Console Software
GeneGo Metacore
TIGR MultiExperiment Viewer
GeneGo
bioconductor
LIMMA
R
Cluster Affinity

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