Changes in expression of the nitric oxide synthase isoforms in rat uterus and cervix during pregnancy and parturition

Molecular Human Reproduction
M AliR E Garfield

Abstract

Nitric oxide (NO) is considered to be an important local mediator that suppresses uterine contractility in rats and rabbits during pregnancy until term. The aim of this study was to investigate the mRNA concentrations for the three isoforms of nitric oxide synthase (NOS) in rat uterus and cervix and to determine whether alterations occur in association with labour at term or preterm. RNA was isolated from full thickness uterine and cervical tissues from pregnant rats at various times during gestation, during labour at term or preterm and post partum. RNA was analysed using reverse transcription-polymerase chain reaction (RT-PCR) with a single set of amplimers specifically designed to detect all three isoforms of NOS. Three distinct PCR products were detected which corresponded to the expected sizes for endothelial (e)NOS, neuronal (b)NOS and inducible (i)NOS products (805, 521 and 428 bp respectively). In all tissues, the 428 bp product predominated and sequence analysis revealed this to be iNOS mRNA with a very close homology (97%) to the published sequence of rat iNOS. Densitometric analysis showed that uterine iNOS mRNA was increased during pregnancy, decreased on day 22 before labour and decreased further during labour at t...Continue Reading

Citations

Jan 4, 2008·Amino Acids·M A GrilloS Colombatto
Apr 7, 2005·Journal of Reproductive Immunology·Verónica L BosquiazzoEnrique H Luque
Nov 14, 2003·Biology of Reproduction·Hyukjung KwonThomas E Spencer
Jan 22, 2005·Biology of Reproduction·Olivier W H van der HeijdenGuillaume J J M van Eys
Dec 11, 2007·Canadian Journal of Physiology and Pharmacology·Abdel-Motaal M FoudaGamal M Dahab
Dec 12, 2012·Contraception·Paula H BednarekJeffrey T Jensen
Jun 26, 2012·Research in Veterinary Science·Hiromichi TamadaTsutomu Sawada
Dec 16, 2006·The Journal of Clinical Hypertension·Thomas D Giles
Jan 10, 2012·Molecular Reproduction and Development·Camila Fernandes MoroPaulo Pinto Joazeiro
Nov 15, 2001·Seminars in Perinatology·R Bukowski, G R Saade
Jan 3, 2006·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Nele FreerksenRobert Garfield
Jun 21, 2015·Der Chirurg; Zeitschrift für alle Gebiete der operativen Medizen·F Stelzner
Jun 13, 2014·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Laura NanettiArianna Vignini
Aug 12, 1999·Nitric Oxide : Biology and Chemistry·I AthanassakisS Vassiliadis
Nov 22, 2001·American Journal of Obstetrics and Gynecology·E G SheselyW H Beierwaltes
Jan 4, 2006·American Journal of Obstetrics and Gynecology·Yuri P VedernikovRobert E Garfield
Apr 23, 2005·American Journal of Obstetrics and Gynecology·Bryan F MitchellSandra T Davidge
Aug 24, 1999·American Journal of Obstetrics and Gynecology·A SyalR E Garfield
Feb 9, 2005·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·T OkawaR E Garfield
Nov 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C N MowaR Papka
Jan 10, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Stephen G MarxRobert E Garfield
Dec 6, 2005·Journal of the Society for Gynecologic Investigation·Laura S KirbySteven M Yellon
Jun 12, 2018·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Lícia Vasconcelos Carvalho da SilvaSophie Helena Eickmann
Dec 31, 2004·Obstetrics and Gynecology·Ambros HuberChristian F Singer
Sep 13, 2006·Molecular Reproduction and Development·Marian AalbertsVidya N A Breeveld-Dwarkasing
May 5, 2005·American Journal of Physiology. Endocrinology and Metabolism·Bryan F MitchellJana M Mitchell
May 8, 2009·The Journal of Veterinary Medical Science·Takehito SuzukiTatsuya Takizawa
Feb 11, 2005·Journal of Physiology and Biochemistry·A GonzálezN López-Moratalla
Oct 8, 2009·Bioscience, Biotechnology, and Biochemistry·Takehito SuzukiTatsuya Takizawa
Aug 16, 2005·Acta Obstetricia Et Gynecologica Scandinavica·Andrea L TranquilliMarta Menegazzi
Sep 24, 2004·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·C N Mowa, R E Papka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.