Changes in FAD and NADH fluorescence in neurosecretory terminals are triggered by calcium entry and by ADP production.

The Journal of Membrane Biology
P KosterinB M Salzberg

Abstract

We measured changes in the intrinsic fluorescence (IF) of the neurosecretory terminals of the mouse neurohypophysis during brief (1-2 s) trains of stimuli. With fluorescence excitation at either 350 +/- 20 or 450 +/- 50 nm, and with emission measured, respectively, at 450 +/- 50 or > or = 520 nm, DeltaF/F(o) was approximately 5-8 % for a 2 s train of 30 action potentials. The IF changes lagged the onset of stimulation by approximately 100 ms and were eliminated by 1 microM tetrodotoxin (TTX). The signals were partially inhibited by 500 microM Cd(2+), by substitution of Mg(2+) for Ca(2+), by Ca(2+)-free Ringer's with 0.5 mM EGTA, and by 50 microM ouabain. The IF signals were also sensitive to the mitochondrial metabolic inhibitors CCCP (0.3 microM), FCCP (0.3 microM), and NaN(3) (0.3 mM), and their amplitude reflected the partial pressure of oxygen (pO(2)) in the bath. Resting fluorescence at both 350 nm and 450 nm exhibited significant bleaching. Flavin adenine dinucleotide (FAD) is fluorescent, while its reduced form FADH(2) is relatively non-fluorescent; conversely, NADH is fluorescent, while its oxidized form NAD is non-fluorescent. Thus, our experiments suggest that the stimulus-coupled rise in [Ca(2+)](i) triggers an incre...Continue Reading

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Citations

Oct 13, 2009·Journal of Fluorescence·Kazuki AitaHideo Saji
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit K ChouhanGregory T Macleod
Jan 7, 2009·Journal of Biomedical Optics·Joseph Foley, Martin Muschol
Feb 18, 2016·The Journal of General Physiology·Shane M McMahonMeyer B Jackson
Apr 30, 2013·Journal of Neuroscience Methods·Vassiliy TsytsarevDmitri B Papkovsky
Feb 23, 2008·Neuroscience Research·Yamato KubotaKatsuei Shibuki
Feb 20, 2007·Biophysical Journal·Bob Eisenberg
Oct 5, 2013·Toxicology and Applied Pharmacology·Jiajun XuStephen R Thom
Mar 5, 2013·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Vânia R Leite-SilvaMichael S Roberts
Oct 9, 2012·Neuron·Anthony N van den Pol
Sep 9, 2016·Journal of Neurophysiology·Brendan LujanRobert Renden
Oct 11, 2013·Journal of Neurophysiology·Daniel A LlanoKevin A Stebbings
Jun 15, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Lilia Coronato Courrol, Marcelo Afonso Vallim

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