Changes in fidelity levels of DNA polymerases alpha-1, alpha-2, and beta during ageing in rats

Mechanisms of Ageing and Development
T Taguchi, M Ohashi

Abstract

DNA polymerases (deoxynucleoside-triphosphate:DNA deoxynucleotidyltransferase EC 2.7.7.7.) were extracted from the regenerating livers of rats of various ages. The extracts were separated into three DNA polymerase fractions (alpha-1, alpha-2, and beta) by phosphocellulose column chromatography, and their fidelity levels were then monitored with the synthetic template-primer, poly (dA-dT), poly dA-dT10, or poly dC-poly dG. The fidelity levels of the three DNA polymerases from regenerating liver of rats younger than 20 months were high, while those of DNA polymerases from rats older than 20 months were significantly lower with similar profiles on all three template-primers. On the other hand, the fidelity levels of enzymes from 23- and 26-month-old rats were similar. These results indicate that the levels of error-prone DNA polymerases increase rapidly in the regenerating liver of rats from ages 20 to 23 months. This may due to the amplification of DNA polymerase gene mutations by an error-prone enzyme itself. However, the cells in which mutations in the functional gene occur may undergo cell death because the fidelity levels of the DNA polymerases in the older animals did not increase.

References

Jun 29, 1978·Biochemical and Biophysical Research Communications·M OhashiS Ikegami
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·G VillaniM Radman
Jan 26, 1978·Biochimica Et Biophysica Acta·J G SalisburyR Saffhill
Sep 1, 1975·Mechanisms of Ageing and Development·M Rothstein
Aug 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·S LinnR Holliday
Jan 1, 1991·Experimental Gerontology·V K SrivastavaD L Busbee
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J HolmesP Modrich
Oct 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·I VarletP Brooks
May 31, 1990·Nature·C B HarleyC W Greider
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·C G FragaB N Ames
Jul 14, 1989·Science·R S LahueP Modrich
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·T InamizuT Makinodan
Jun 1, 1986·Microbiological Reviews·J P Claverys, S A Lacks
Mar 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·H Gershon, D Gershon
Jan 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·C F Springgate, L A Loeb
Sep 28, 1968·Journal of Molecular Biology·Z W Hall, I R Lehman
May 1, 1982·Mechanisms of Ageing and Development·A A MorleyR Holliday
Nov 24, 1995·Mechanisms of Ageing and Development·S JinnoT Taguchi
Jul 7, 1972·Nature·R Holliday, G M Tarrant
Mar 15, 1962·Proceedings of the National Academy of Sciences of the United States of America·T A TRAUTNERA KORNBERG
Jan 1, 1959·Proceedings of the National Academy of Sciences of the United States of America·L Szilard

❮ Previous
Next ❯

Citations

Dec 31, 2003·Experimental Gerontology·Vinod K Srivastava, David L Busbee
Nov 12, 2002·Mechanisms of Ageing and Development·Takao KanekoTakahiko Taguchi
Jan 28, 1999·Mechanisms of Ageing and Development·T TaguchiM Ohashi
Jan 12, 1999·Mechanisms of Ageing and Development·T TaguchiM Ohashi
Oct 9, 1999·Mechanisms of Ageing and Development·M FukudaM Ohashi
Oct 19, 2002·Critical Reviews in Clinical Laboratory Sciences·Gunther GuetensErnst A de Bruijn
Feb 5, 2003·Experimental and Molecular Pathology·Mari OgiharaTakahiko Taguchi

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
J Y Chan, F F Becker
Proceedings of the National Academy of Sciences of the United States of America
A MatsukageS H Wilson
Proceedings of the National Academy of Sciences of the United States of America
R W HendlerR Scharff
© 2021 Meta ULC. All rights reserved