Changes in Fourier transform infrared spectra of Salmonella enterica serovars Typhimurium and Enteritidis after adaptation to stressful growth conditions

International Journal of Food Microbiology
Avelino Alvarez-OrdóñezM Prieto

Abstract

The effects of growth conditions (temperature in the range 10-45 degrees C, sodium chloride concentration in the range 0-4%, aerobic vs. anaerobic growth and acidification of the growth medium, up to pH 4.5) on the Fourier transform infrared (FT-IR) spectra of Salmonella enterica serovars Typhimurium and Enteritidis were studied using multivariate statistical methods (Hierarchical Cluster Analysis and Factor Analysis). Although all environmental factors tested affected S. Typhimurium and S. Enteritidis FT-IR spectra to some extent, growth temperature was the most influential factor within the five spectral regions. The w(4) spectral region (1200 to 900 cm(-1)) was the most variable region, suggesting that S. Typhimurium and S. Enteritidis modulate their cell wall and cell membrane composition in response to shifts in growth temperature. Changes in membrane fluidity were determined by monitoring the vibrational modes of the acyl chain v(s)CH(2) symmetric stretching band by FT-IR spectroscopy. For cells grown in unsupplemented media an increase in growth temperature was linked to a decrease in membrane fluidity. Even though the effect of NaCl concentration, pH and atmosphere was considered of less importance, cells grown in acidi...Continue Reading

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Citations

Jan 17, 2012·Applied and Environmental Microbiology·Benoît de SarrauChristophe Nguyen-The
Jan 15, 2014·International Journal of Food Microbiology·Akanksha Gandhi, Nagendra P Shah
May 31, 2014·Fish Physiology and Biochemistry·Ekambaram Padmini, Jayachandran Tharani
Apr 16, 2013·Journal of Applied Microbiology·A Álvarez-OrdóñezP Rubio
Jun 28, 2015·International Journal of Food Microbiology·Avelino Alvarez-OrdóñezMiguel Prieto
Oct 22, 2011·Microbiology·Avelino Álvarez-OrdóñezColin Hill
Feb 23, 2020·Frontiers in Microbiology·Chloé ModugnoJean-Marie Perrier-Cornet
Oct 8, 2021·Frontiers in Microbiology·Marta Clemente-CarazoPaula M Periago
Nov 4, 2021·Applied Microbiology and Biotechnology·K G MladenovićS D Kocić-Tanackov

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