Abstract
Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-mycelium conversion. The reason for the decline of glutathione in the mycelial form is not understood. We have, therefore, investigated the levels of glutathione reductase, glutathione S-transferase, gamma-glutamyltranspeptidase, and glutathione peroxidase, four key enzymes involved in glutathione metabolism, in the yeast and mycelial forms. Yeast cells of C. albicans 3153A were induced in Lee's medium (pH 6.5) at 37 degrees C for 3 h to produce germ tubes. Cell lysates were prepared from yeast and mycelial cells, and glutathione reductase, glutathione S-transferase, gamma-glutamyltranspeptidase, and glutathione peroxidase were assayed spectrophotometrically. There was a 640% increase of the level of gamma-glutamyltranspeptidase in the germ tubes as compared with the yeast cells. No other significant alteration of the levels of enzymes was noted. This increased activity of gamma-glutamyltranspeptidase, which cleaves the g...Continue Reading
References
Jul 1, 1975·Sabouraudia·K L LeeC C Campbell
Jan 1, 1985·Annual Review of Biochemistry·D M Ziegler
Jan 1, 1985·Methods in Enzymology·M WarholmB Mannervik
Jan 1, 1985·Methods in Enzymology·M E Anderson
Jan 1, 1981·Methods in Enzymology·A MeisterO W Griffith
Jun 1, 1984·Infection and Immunity·J D SobelH R Buckley
Oct 1, 1983·Journal of General Microbiology·J C AhrensH R Buckley
Jul 1, 1984·Infection·T SöderströmL A Hanson
Jan 1, 1984·Methods in Enzymology·L Flohé, W A Günzler
Citations
Nov 1, 2001·Journal of Immunological Methods·C G HaidarisM A Sullivan
May 25, 2004·Antimicrobial Agents and Chemotherapy·Joseph M BlissConstantine G Haidaris
Mar 8, 2003·Journal of Clinical Microbiology·Joseph M BlissConstantine G Haidaris
Mar 22, 2005·Biochimica Et Biophysica Acta·Pilar González-PárragaJosé A Hernández
Oct 6, 2005·Journal of Biochemistry and Molecular Biology·Hyun-Jung KangChang-Jin Lim
Apr 17, 2016·Biochimica Et Biophysica Acta·R GergondeyF Auchère
Jul 16, 2014·Biochimica Et Biophysica Acta·H GuedouariF Auchère
Jul 12, 2002·Acta Microbiologica Et Immunologica Hungarica·T EmriI Pócsi
Jan 17, 2002·Acta Microbiologica Et Immunologica Hungarica·I PócsiT Pusztahelyi
Feb 19, 2008·Acta Biologica Hungarica·I PócsiT Emri
Aug 26, 2009·FEMS Yeast Research·Carmen Michán, Carmen Pueyo
Feb 22, 2007·FEMS Yeast Research·Joël CoulonPierre Leroy
Apr 19, 2003·FEMS Yeast Research·Michel J Penninckx
Mar 31, 2004·Canadian Journal of Microbiology·Hey-Jung ParkChang-Jin Lim
Oct 31, 2003·Bioscience, Biotechnology, and Biochemistry·Tsuyoshi MiyakeBun-ichiro Ono
Jan 17, 2002·Acta Microbiologica Et Immunologica Hungarica·I PócsiA Szentirmai
Jul 10, 2001·Journal of Basic Microbiology·C W JürgensenI Pócsi
May 28, 2005·Canadian Journal of Microbiology·Hey-Jung ParkChang-Jin Lim