Changes in glutathione metabolic enzymes during yeast-to-mycelium conversion of Candida albicans

Canadian Journal of Microbiology
M ManavathuM Gunasekaran

Abstract

Candida albicans is a dimorphic yeast capable of producing alternate morphological forms (yeast or mycelium) in response to environmental changes. The intracellular level of glutathione, which helps to maintain the redox potential of the cell, is decreased significantly during the thermal induction of yeast-to-mycelium conversion. The reason for the decline of glutathione in the mycelial form is not understood. We have, therefore, investigated the levels of glutathione reductase, glutathione S-transferase, gamma-glutamyltranspeptidase, and glutathione peroxidase, four key enzymes involved in glutathione metabolism, in the yeast and mycelial forms. Yeast cells of C. albicans 3153A were induced in Lee's medium (pH 6.5) at 37 degrees C for 3 h to produce germ tubes. Cell lysates were prepared from yeast and mycelial cells, and glutathione reductase, glutathione S-transferase, gamma-glutamyltranspeptidase, and glutathione peroxidase were assayed spectrophotometrically. There was a 640% increase of the level of gamma-glutamyltranspeptidase in the germ tubes as compared with the yeast cells. No other significant alteration of the levels of enzymes was noted. This increased activity of gamma-glutamyltranspeptidase, which cleaves the g...Continue Reading

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Citations

Nov 1, 2001·Journal of Immunological Methods·C G HaidarisM A Sullivan
May 25, 2004·Antimicrobial Agents and Chemotherapy·Joseph M BlissConstantine G Haidaris
Oct 6, 2005·Journal of Biochemistry and Molecular Biology·Hyun-Jung KangChang-Jin Lim
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May 28, 2005·Canadian Journal of Microbiology·Hey-Jung ParkChang-Jin Lim

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