Changes in hepatic gene expression related to innate immunity, growth and iron metabolism in GH-transgenic amago salmon (Oncorhynchus masou) by cDNA subtraction and microarray analysis, and serum lysozyme activity

General and Comparative Endocrinology
Tsukasa MoriKazuo Araki

Abstract

Growth hormone (GH) transgenic amago salmon (Oncorhynchus masou) were generated with a construct containing the sockeye salmon GH1 gene fused to the metallothionein-B (MT-B) promoter from the same species. This transgene directed significant growth enhancement with transgenic fish reaching approximately four to five times greater weight than control salmon in F(2) and F(3) generations. This drastic growth enhancement by GH transgene is well known in fish species compared with mammals, however, such fish can show morphological abnormalities and physiological disorders like other GH transgenic animals. GH is known to have many acute effects, but currently there are no data describing the chronic effects of over-expression of GH on various hepatic genes in GH transgenic fish. Hepatic gene expression is anticipated to play very important roles in many physiological functions and growth performance of transgenic and control salmon. To examine these effects, we performed subtractive hybridization (using cDNA generated from liver RNA) in both directions to identify genes both increased and decreased in transgenic salmon relative to controls (576 clones were isolated and sequenced in total). Heme oxygenase, vitelline envelope protein, ...Continue Reading

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Citations

Feb 19, 2009·Proceedings of the National Academy of Sciences of the United States of America·Robert H DevlinBenjamin Goh
Dec 31, 2011·Journal of Proteomics·Yoichi KurataTsukasa Mori
Dec 20, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Jiong ChenMing Y Li
Nov 5, 2013·General and Comparative Endocrinology·Mengxi CaoWei Hu
Nov 14, 2008·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Ryan A DombkowskiKenneth R Olson
Jul 26, 2021·Transgenic Research·Arinze S OkoliXiaodong Xu

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