Changes in human donor corneas preserved for longer than 4 weeks

Cornea
C RedbrakeA Frantz

Abstract

Corneas are usually stored for a maximum of approximately 30 days in European cornea banks. Although attempts are being made to prolong culture periods, data on their success are extremely limited to date. The following study was carried out to describe the capacities and limits of the established system. Thirty-seven human corneas were stored for < or = 12 weeks under standard eye bank conditions [modified minimal essential medium (MEM), 31 degrees C, closed system]. Twenty-one fresh human corneas served as control. Both the adenylate nucleotides and the glucose and lactate concentrations were measured in the tissue (all cellular layers) by using the bioluminescence technique. The endothelial-cell densities also were determined. Endothelial-cell densities decreased from 2,963.4 +/- 58.7 cells/mm2 (fresh) to 2,649 cell/mm2 after 4 weeks and to 2,087 cells/mm2 after 6 weeks. Storage for periods >6 weeks led to total endothelial necrosis. Biochemical studies showed improving values during the first 4 weeks and acceptable conditions for < or = 6 weeks. From these data, we conclude that long-term organ culture in a closed system is limited to approximately 6 weeks and thereby confirm the clinical results of Früh and Böhnke.

Citations

Sep 24, 2009·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Martin HermelPeter Walter
Sep 21, 2000·Transplantation Proceedings·J SzaflikM Brix
Jul 27, 2001·Cornea·W M Bourne
May 8, 2009·Cornea·Armin H WolfAljoscha S Neubauer
Aug 25, 2001·The British Journal of Ophthalmology·T Møller-PedersenK Engelmann
Aug 14, 2012·Clinical Ophthalmology·Armin WolfMartin Grueterich
Aug 21, 2010·Eye & Contact Lens·William M Bourne
Aug 11, 2006·Current Opinion in Ophthalmology·Bennie H Jeng
Sep 22, 2011·Acta Ophthalmologica·Tomasz KryczkaAnna Midelfart
Oct 26, 2005·Journal of Cataract and Refractive Surgery·Madhavan S RajanJohn Marshall
Feb 22, 2011·American Journal of Ophthalmology·Kathrin LaaserFriedrich E Kruse
Aug 21, 2016·The British Journal of Ophthalmology·Maninder BhogalBruce D Allan
Jul 3, 2018·Cornea·Aida Madzak, Jesper Hjortdal
Jan 3, 2020·Transplantation·Thibaud GarcinGilles Thuret

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