Changes in human lymphocyte subpopulations in tonsils and regional lymph nodes of human head and neck squamous carcinoma compared to control lymph nodes

BMC Immunology
B Vidal-RubioF Gambón-Deza

Abstract

Lymphoid tissues constitute basic structures where specific immune responses take place. This leads to the development of germinal centres (GCs), migration of cells and the generation of memory cells. Here, we have compared human tumour reactive lymph nodes and tonsils with control lymph nodes. The study by flow cytometry shows that in control lymph nodes the majority of cells were naive T-lymphocytes (CD45RA+/CD7+). In reactive nodes, although the percentage of CD45RO+ T cells remains constant, there is an increase in the number of B-lymphocytes, and a reduction in naive T cells. The percentage of cells expressing CD69 was similar in reactive nodes and in controls. In both cases, we have found two populations of B cells of either CD69- or CD69dull. Two populations of T cells, which are either negative for CD69 or express it in bright levels (CD69bright), were also found. The analysis of tissue sections by confocal microscopy revealed differences between control, tonsils and tumor reactive lymph nodes. In control lymph nodes, CD19 B cells are surrounded by a unique layer of CD69bright/CD45RO+ T cells. GCs from tonsils and from tumour reactive nodes are mainly constituted by CD19 B cells and have four distinct layers. The centra...Continue Reading

References

Jan 1, 1991·Veterinary Immunology and Immunopathology·C R MackayW R Hein
Jan 1, 1989·European Journal of Immunology·R Testi, L L Lanier
Apr 5, 1997·Annals of the New York Academy of Sciences·A Gulbranson-JudgeI C MacLennan
Jan 12, 2002·Developmental Immunology·W J SimmonsG J Thorbecke

❮ Previous
Next ❯

Citations

Sep 7, 2017·PloS One·Katharina GeißlerOrlando Guntinas-Lichius
Feb 23, 2021·ACS Pharmacology & Translational Science·Maura C BelangerRebecca R Pompano

❮ Previous
Next ❯

Methods Mentioned

BETA
confocal microscopy
flow cytometry
confocal
FCS

Software Mentioned

SPSS

Related Concepts

Related Feeds

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.

Related Papers

Zeitschrift für Zellforschung und mikroskopische Anatomie
E Kaiserling, R Caesar
Advances in Experimental Medicine and Biology
T Defrance, C Lagresle
Archiv für klinische und experimentelle Ohren- Nasen- und Kehlkopfheilkunde
W Behrendt
© 2021 Meta ULC. All rights reserved