PMID: 11342258May 9, 2001Paper

Changes in hydration, protein and proteoglycan composition of the collagen-keratocyte matrix of the bovine corneal stroma ex vivo in a bicarbonate-mixed salts solution, compared to other solutions

Biochimica Et Biophysica Acta
Michael J Doughty

Abstract

Many solutions have been used to investigate the swelling properties of the mammalian corneal stroma but few of the solutions resemble the expected extracellular matrix fluid of the corneal stroma, and little information is available on whether incubation ex vivo causes significant changes in the gross composition of the stroma. From quality-selected recent post-mortem eyes of adult cattle, stroma preparations were cut from the central part of the cornea. The time-dependent changes in wet mass were assessed over 9 h at 37 degrees C, and the preparations then dried. Various solutions of known pH (6.88-8.32) and osmolality (<50-327 mosmol/kg) were used, and were assayed for protein and proteoglycan after the incubation. The rates and extent of stromal swelling were lowest in a glucose-supplemented mixed salts solution containing 35 mM bicarbonate (0.5% CO2) solution, marginally greater in a mixed salts solution containing 35 mM bicarbonate (5% CO2) or similar non-bicarbonate mixed salts solutions (including BSS), and progressively greater in phosphate-buffered saline (PBS), various phosphate buffers (10-67 mM) and saline solutions (0.025-1%), and greatest in water. The initial rates of swelling ranged from 44 to 451 mg/h and the ...Continue Reading

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Citations

Nov 24, 2005·Contact Lens & Anterior Eye : the Journal of the British Contact Lens Association·Michael J Doughty
Nov 21, 2015·Tissue Engineering. Part C, Methods·Jie ZhangDipika V Patel
Dec 1, 2001·Human Reproduction·M R LuckA Cooper
Jun 21, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Shukti ChakravartiDavid E Birk

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