Changes in immune cell frequencies in primary and secondary lymphatic organs of LEW.1AR1-iddm rats, a model of human type 1 diabetes compared to other MHC congenic LEW inbred strains

Immunologic Research
Tanja ArndtDirk Wedekind

Abstract

The LEW.1AR1-iddm rat is an animal model of human type 1 diabetes, which arose through a spontaneous mutation in the Dock8 gene within the MHC congenic background strain LEW.1AR1. This mutation not only mediates diabetes development but also leads to a variable T cell frequency in peripheral blood. In this study, the immune cell frequencies of primary and secondary lymphatic organs of LEW.1AR1-iddm rats were analysed at days 40 and 60 and compared to other MHC congenic LEW rat strains. In LEW.1AR1-iddm rats, the secondary lymphatic organs such as lymph nodes and spleen showed a reduced, around 15% in comparison to all other strains, but very variable T cell frequency, mirroring the fluctuating T cell content in blood. On the other hand, the frequency of B cells was increased by 10% in the lymph nodes and by 5% in the spleen. Thus, the decreasing number of T cells in blood could not be caused by an increase of T cells in secondary lymphatic organs. The frequency of single- or double-positive T cells in the thymus was unaffected. The T cell frequencies in the other analysed strains were more stable and mostly higher in all secondary lymphatic organs. Obviously, the Dock8 mutation leads to variabilities of T cell frequencies in bl...Continue Reading

References

Oct 1, 1981·Diabetes·R JacksonG S Eisenbarth
Jun 28, 2002·Immunological Reviews·S Ramanathan, P Poussier
Jul 16, 2002·Nature Genetics·Norihide YokoiSusumu Seino
Jul 2, 2004·ILAR Journal·John P MordesDale L Greiner
Aug 3, 2005·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Heike WeissDirk Wedekind
Nov 30, 2006·Immunology Letters·Katrin S Blum, Reinhard Pabst
Mar 22, 2008·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Heike WeissDirk Wedekind
Sep 25, 2009·The New England Journal of Medicine·Qian ZhangHelen C Su
Mar 2, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Michael J BarnesKasper Hoebe
Oct 5, 2011·European Journal of Immunology·Teresa LambeRichard J Cornall
May 15, 2012·Nature Immunology·Haifa H JabaraRaif S Geha
Feb 6, 2013·The Journal of Allergy and Clinical Immunology·Melissa C MizeskoJordan S Orange
Mar 5, 2013·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hyoungjun HamDaniel D Billadeau
Feb 6, 2014·Laboratory Animals·UNKNOWN FELASA working group on revision of guidelines for health monitoring of rodents and rabbitsM Raspa
Feb 26, 2015·Proceedings of the National Academy of Sciences of the United States of America·Jayendra Kumar KrishnaswamyStephanie C Eisenbarth
May 13, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Mirkka T HeinonenUNKNOWN Finnish Pediatric Diabetes Registry

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Methods Mentioned

BETA
FACS
flow cytometry
GTPase
GTPases

Software Mentioned

GraphPad Prism

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