Changes in intestinal barrier function and gut microbiota in high-fat diet-fed rats are dynamic and region dependent

American Journal of Physiology. Gastrointestinal and Liver Physiology
M Kristina HamiltonHelen E Raybould

Abstract

A causal relationship between the pathophysiological changes in the gut epithelium and altered gut microbiota with the onset of obesity have been suggested but not defined. The aim of this study was to determine the temporal relationship between impaired intestinal barrier function and microbial dysbiosis in the small and large intestine in rodent high-fat (HF) diet-induced obesity. Rats were fed HF diet (45% fat) or normal chow (C, 10% fat) for 1, 3, or 6 wk; food intake, body weight, and adiposity were measured. Barrier function ex vivo using FITC-labeled dextran (4,000 Da, FD-4) and horseradish peroxidase (HRP) probes in Ussing chambers, gene expression, and gut microbial communities was assessed. After 1 wk, there was an immediate but reversible increase in paracellular permeability, decrease in IL-10 expression, and decrease in abundance of genera within the class Clostridia in the ileum. In the large intestine, HRP flux and abundance of genera within the order Bacteroidales increased with time on the HF diet and correlated with the onset of increased body weight and adiposity. The data show immediate insults in the ileum in response to ingestion of a HF diet, which were rapidly restored and preceded increased passage of l...Continue Reading

References

Jul 22, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ruth E LeyJeffrey I Gordon
Oct 7, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Paola BrunDiego Martines
Dec 22, 2006·Nature·Ruth E LeyJeffrey I Gordon
Dec 22, 2006·Nature·Peter J TurnbaughJeffrey I Gordon
Jan 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Fredrik BäckhedJeffrey I Gordon
Oct 16, 2007·Clinical Microbiology Reviews·Hannah M Wexler
Jan 17, 2008·Journal of Cell Science·Christina M Van ItallieJames M Anderson
Sep 26, 2008·Journal of Lipid Research·Sarbani GhoshalErik Eckhardt
Feb 5, 2009·American Journal of Physiology. Endocrinology and Metabolism·Gabriel PaulinoHelen E Raybould
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
May 29, 2010·American Journal of Physiology. Gastrointestinal and Liver Physiology·Claire Barbier de La SerreHelen E Raybould
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·J Gregory CaporasoRob Knight
Aug 21, 2010·Inflammatory Bowel Diseases·Sa'ad Y Salim, Johan D Söderholm
Oct 28, 2010·Nutrition Research Reviews·Niloofar Hariri, Louise Thibault
Jan 14, 2011·Annual Review of Medicine·Giovanni MussoMaurizio Cassader
Feb 8, 2011·Lancet·Mariel M FinucaneUNKNOWN Global Burden of Metabolic Risk Factors of Chronic Diseases Collaborating Group (Body Mass Index)
Feb 10, 2011·Standards in Genomic Sciences·Jack A GilbertRick Stevens
Jun 3, 2011·The Journal of Clinical Investigation·Herbert Tilg, Arthur Kaser
Jun 28, 2011·Genome Biology·Nicola SegataCurtis Huttenhower
Dec 21, 2011·The Journal of Physiology·Helen E Raybould
Jan 10, 2012·Annual Review of Immunology·Kenya Honda, Dan R Littman
Mar 27, 2012·Clinical Nutrition : Official Journal of the European Society of Parenteral and Enteral Nutrition·Tatiana F S TeixeiraMaria do Carmo G Peluzio
Oct 24, 2012·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Jane M M Natividad, Elena F Verdu
Nov 30, 2012·The ISME Journal·Sebastian TimsErwin G Zoetendal
Feb 6, 2013·Current Opinion in Gastroenterology·Kathryn A KnoopRodney D Newberry
Mar 29, 2013·Science Translational Medicine·Alice P LiouLee M Kaplan
May 15, 2013·Biochimica Et Biophysica Acta·Shahzad SiddiqiCharles M Mansbach
May 15, 2013·Proceedings of the National Academy of Sciences of the United States of America·Amandine EverardPatrice D Cani
Sep 14, 2013·The ISME Journal·Hannelore DanielThomas Clavel
Dec 18, 2013·Nature·Lawrence A DavidPeter J Turnbaugh

❮ Previous
Next ❯

Citations

Feb 16, 2016·Microbial Pathogenesis·Muriel DerrienWillem M de Vos
Feb 9, 2016·Cell Metabolism·Daniel A WinerShawn Winer
Jul 18, 2015·Molecular Nutrition & Food Research·Yadong ZhongFrida Fåk
Dec 19, 2015·Molecular Nutrition & Food Research·Veronika Maria MüllerHannelore Daniel
Jun 19, 2016·Lipids in Health and Disease·Othman A BaothmanMohamed Abu-Farha
Aug 16, 2016·Translational Research : the Journal of Laboratory and Clinical Medicine·Zora Djuric
Aug 26, 2016·Nutrition & Metabolism·Maria KuleckaJerzy Ostrowski
Sep 18, 2016·Proceedings of the National Academy of Sciences of the United States of America·Julie TomasThierry Pédron
Oct 13, 2016·Journal of Animal Physiology and Animal Nutrition·S GilaniR J Hughes
Oct 30, 2016·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·Y LiuH Gregersen
Feb 25, 2017·Cell·Christoffer ClemmensenMatthias H Tschöp
Feb 22, 2017·Molecular Nutrition & Food Research·Katherine Gil-CardosoXimena Terra
Oct 27, 2016·Exercise and Sport Sciences Reviews·Sara C Campbell, Paul J Wisniewski
Jun 7, 2017·Gut Microbes·Burkhardt FlemerJean-François Brugère
Jun 27, 2017·Expert Review of Gastroenterology & Hepatology·Maaike Vancamelbeke, Séverine Vermeire
Mar 3, 2017·Journal of Diabetes Research·Chang XiaJixin Zhong
Jul 2, 2016·Therapeutic Advances in Gastroenterology·Justin L McCarvilleElena F Verdu
Dec 6, 2018·Nutrition Reviews·Maude Le GallJohanne Le Beyec
Nov 22, 2018·Developmental Psychobiology·Jing Lu, Erika C Claud
May 28, 2019·American Journal of Physiology. Gastrointestinal and Liver Physiology·Michael CamilleriGary D Wu
Jul 4, 2019·Advances in Nutrition·Michael W RohrSampath Parthasarathy
Aug 8, 2015·American Journal of Physiology. Gastrointestinal and Liver Physiology·Emily A SawinDenise M Ney
Apr 13, 2017·American Journal of Physiology. Endocrinology and Metabolism·Mathilde GuervilleGaëlle Boudry
Sep 27, 2019·The Journal of Spinal Cord Medicine·Gregory M HolmesNaomi Kleitman
Oct 4, 2017·The Journal of Clinical Endocrinology and Metabolism·Yuan-Cheng ChenHong-Wen Deng
Jan 18, 2018·Journal of Food Science·Gessika Cristina Borges Castro CarvalhoJaime Amaya-Farfan
Jan 25, 2018·Obesity Reviews : an Official Journal of the International Association for the Study of Obesity·R J MuldersR A H Adan
Sep 13, 2019·Journal of the Science of Food and Agriculture·Ying GaoJunfeng Yin
Feb 27, 2018·Journal of Alzheimer's Disease : JAD·Leszek Szablewski
Dec 31, 2019·MicrobiologyOpen·Yajing Song, Peter Gyarmati
Mar 4, 2020·European Journal of Nutrition·Moon Ho DoHo-Young Park

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cardiovascular Diseases: Risk Factors

Cardiovascular disease is a significant health concern. Risk factors include hypertension, obesity, dyslipidemia and smoking. Women who are postmenopausal are at an increased risk of heart disease. Here is the latest research for risk factors of cardiovascular disease.