Changes in intestinal microbiota of Bufo gargarizans and its association with body weight during metamorphosis

Archives of Microbiology
Lihong ChaiHongyuan Wang

Abstract

The assembly of intestinal microbial communities can play major roles in animal development. We hypothesized that intestinal microbial communities could mirror the developmental programs of amphibian metamorphosis. Here, we surveyed the morphological parameters of the body and intestine of Bufo gargarizans at varying developmental stages and inventoried the intestinal microbial communities of B. gargarizans at four key developmental stages via 16S rDNA gene sequencing. Firstly, our survey showed that during metamorphosis, body weight and intestinal weight were reduced by 56.8 and 91.8%, respectively. Secondly, the gut bacterial diversity of B. gargarizans decreased with metamorphosis and the composition of the tadpoles' intestinal microbiota varied across metamorphosis. Compared to aquatic larvae, terrestrial juveniles showed major shifts in microbial composition, including reduction in Proteobacteria and Actinobacteria, increases in Bacteroidetes and Fusobacteria, and the appearance of Verrucomicrobia. Firmicutes in four developmental stages showed similar abundance at the phylum level, but in each stage was driven by distinct genera. Enterobacter, Aeromonas, Mucinivorans and Bacteroides also changed in abundance and were foun...Continue Reading

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Citations

Apr 12, 2020·Journal of Applied Microbiology·Xin MengHao Wu
Jan 29, 2020·Molecular Ecology Resources·Jia ZhouLee Ann Rollins
May 15, 2021·The Journal of Experimental Biology·Jessica A CusickGregory E Demas

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Datasets Mentioned

BETA
SRP087600

Methods Mentioned

BETA
electrophoresis
PCR
Illumina sequencing

Software Mentioned

Bioconductor
UPARSE
UCHIME
Quantitative Insights Into Microbial Ecology ( QIIME
R
QIIME
vegan
Fast Tree
MOTHUR
Usearch

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