Changes in intestinal microflora of Caenorhabditis elegans following Bacillus nematocida B16 infection

Scientific Reports
Qiuhong NiuLunguang Yao

Abstract

The effect of pathogenic bacteria on a host and its symbiotic microbiota is vital and widespread in the biotic world. The soil-dwelling opportunistic bacterium Bacillus nematocida B16 uses a "Trojan horse" mechanism to kill Caenorhabditis elegans. The alterations in the intestinal microflora that occur after B16 infection remain unknown. Here, we analyzed the intestinal bacteria presented in normal and infected worms. The gut microbial community experienced a complex change after B16 inoculation, as determined through marked differences in species diversity, structure, distribution and composition between uninfected and infected worms. Regardless of the worm's origin (i.e., from soil or rotten fruits), the diversity of the intestinal microbiome decreased after infection. Firmicutes increased sharply, whereas Proteobacteria, Actinobacteria, Cyanobacteria and Acidobacteria decreased to different degrees. Fusobacteria was only present 12 h post-infection. After 24 h of infection, 1228 and 1109 bacterial species were identified in the uninfected and infected groups, respectively. The shared species reached 21.97%. The infected group had a greater number of Bacillus species but a smaller number of Pediococcus, Halomonas, Escherichia...Continue Reading

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Citations

Nov 16, 2019·Microbial Biotechnology·Lin ZhangQiuhong Niu
Oct 5, 2019·Cellular and Molecular Life Sciences : CMLS·Arun KumarMojibur Khan
Jun 25, 2021·Trends in Parasitology·Olivera Topalović, Mette Vestergård
Sep 25, 2021·Archives of Microbiology·Xuyang WeiQiuhong Niu

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Methods Mentioned

BETA
light
transmission electron microscopy

Software Mentioned

UniFrac
MGRAST
Mother
Flash
UCLUST

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