Changes in intracellular Ca2+ concentration induced by L-type Ca2+ channel current in guinea pig gastric myocytes

The American Journal of Physiology
S J KimK W Kim

Abstract

We investigated the relationship between voltage-operated Ca2+ channel current and the corresponding intracellular Ca2+ concentration ([Ca2+]i) change (Ca2+ transient) in guinea pig gastric myocytes. Fluorescence microspectroscopy was combined with conventional whole cell patch-clamp technique, and fura 2 (80 microM) was added to CsCl-rich pipette solution. Step depolarization to 0 mV induced inward Ca2+ current (ICa) and concomitantly raised [Ca2+]i. Both responses were suppressed by nicardipine, an L-type Ca2+ channel blocker, and the voltage dependence of Ca2+ transient was similar to the current-voltage relation of ICa. When pulse duration was increased by up to 900 ms, peak Ca2+ transient increased and reached a steady state when stimulation was for longer. The calculated fast Ca2+ buffering capacity (B value), determined as the ratio of the time integral of ICa divided by the amplitude of Ca2+ transient, was not significantly increased after depletion of Ca2+ stores by the cyclic application of caffeine (10 mM) in the presence of ryanodine (4 microM). The addition of cyclopiazonic acid (CPA, 10 microM), a sarco(endo)plasmic reticulum Ca(2+)-ATPase inhibitor, decreased B value by approximately 20% in a reversible manner. W...Continue Reading

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Citations

Nov 1, 2003·American Journal of Physiology. Cell Physiology·Yong-Xiao WangMichael I Kotlikoff
Jan 21, 2010·Physiological Reviews·Susan Wray, Theodor Burdyga
Jul 8, 2011·American Journal of Physiology. Cell Physiology·Tae Sik SungInsuk So
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Apr 22, 2005·American Journal of Physiology. Cell Physiology·Mei Hong ZhuKi Whan Kim
Nov 10, 2006·Pflügers Archiv : European journal of physiology·Qing-Hua LiuYong-Xiao Wang
May 9, 2007·Annals of Biomedical Engineering·Alberto Corrias, Martin L Buist
Jan 8, 2010·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young Chul KimHyo-Yung Yun
Nov 3, 2012·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Dae Hoon KimSang Jin Lee
Apr 30, 2016·American Journal of Physiology. Gastrointestinal and Liver Physiology·Curtis SobchakJaques Belik
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Apr 1, 2008·The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology·Young Chul KimSang Jin Lee
Feb 14, 2007·Journal of Korean Medical Science·Young Chul KimKi Whan Kim
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