Changes in intracellular Ca2+ induced by GABAA receptor activation and reduction in Cl- gradient in neonatal rat neocortex

Journal of Neurophysiology
A FukudaH Nishino

Abstract

We have studied the effects of gamma-aminobutyric acid (GABA) and of reducing the Cl- gradient on the [Ca2+]i in pyramidal neurons of rat somatosensory cortex. The Cl- gradient was reduced either with furosemide or by oxygen-glucose deprivation. Immature slices taken at postnatal day (P)7-14 were labeled with fura-2, and [Ca2+]i was monitored in identified pyramidal cells in layer II/III as the ratio of fluorescence intensities (RF340/F380). The magnitude of the [Ca2+]i increases induced by oxygen-glucose deprivation was significantly reduced (by 44%) by bicuculline (10 microM), a GABAA receptor antagonist. Under normal conditions, GABA generally did not raise [Ca2+]i, although in some neurons a small and transient [Ca2+]i increase was observed. These transient [Ca2+]i increases were blocked by Ni2+ (1 mM), a blocker of voltage-dependent Ca2+ channels (VDCCs). Continuous perfusion with GABA did not cause a sustained elevation of [Ca2+]i but bicuculline caused [Ca2+]i oscillations. After inhibition of Cl- extrusion with furosemide (1.5 mM), GABA induced a large [Ca2+]i increase consisting of an initial peak followed by a sustained phase. Both the initial and the sustained phases were eliminated by bicuculline (10 microM). The in...Continue Reading

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Citations

Mar 15, 2002·Neuroreport·Hideyuki ToriyamaTsutomu Tanabe
Jan 11, 2003·Journal of Neurophysiology·Stacey L SchombergDandan Sun
Aug 9, 2003·Journal of Neurophysiology·Hao WangDandan Sun
Oct 7, 2015·Frontiers in Cellular Neuroscience·Miho Watanabe, Atsuo Fukuda
Dec 10, 2019·Oxidative Medicine and Cellular Longevity·Chaoran ChenGuangli Lu
Apr 29, 2006·The Journal of Physiology·Sampsa T SipiläKai Kaila
Feb 7, 2013·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Ryuta Koyama
Sep 8, 2007·Developmental Neurobiology·Joseph L Nuñez, Margaret M McCarthy
Apr 23, 2005·Neurological Research·Hai Chen, Dandan Sun
Oct 12, 2007·Physiological Reviews·Yehezkel Ben-AriRustem Khazipov
Sep 5, 2002·Nature Reviews. Neuroscience·Yehezkel Ben-Ari
Jun 7, 2014·The European Journal of Neuroscience·Xiaohong SongMasahiko Watanabe
Aug 29, 2001·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·B HuE Tong
Mar 24, 2006·The European Journal of Neuroscience·Yoshiyuki SakataShoji Nakamura
Feb 5, 2002·Annual Review of Physiology·Eric Delpire, David B Mount
Feb 20, 2013·Brain Structure & Function·Krisztina KovácsAttila Sík
Jul 11, 2002·Brain Research. Molecular Brain Research·Luyan SongDavid B Mount
Apr 12, 2003·Trends in Neurosciences·John A PayneKai Kaila
Mar 28, 2009·Neuron·Peter BlaesseKai Kaila

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