Changes in intracellular calcium concentration and P2X1 receptor expression in hypertrophic rat urinary bladder smooth muscle

Neurourology and Urodynamics
R Sjuve ScottAnders Arner

Abstract

Contractile responses to purinergic activation in the urinary bladder are altered in outflow obstruction (O). We determined if the lowered contractile response to adenosine triphosphate (ATP) in obstructed rat urinary bladder was due to changes in calcium handling or in P2X1 purinoceptor density. O was created in rat by partial ligature of the urethra, with non-obstructed rats as controls (C). Force and intracellular calcium were measured in bladder strips activated with ATP. Tissue was sectioned for light and electron microscopy and analyzed with Western blot using a P2X1 antibody. Bladder weight increased from 66 +/- 3 (C) to 206 +/- 17 mg (O) (n = 6). ATP gave a transient contractile response which was decreased in the obstructed strips (C: 161 +/- 20; O: 63 +/- 16% of high-K+ force). Intracellular calcium concentration after ATP activation in the obstructed bladder muscle was about 50% of that in the control preparations (C: 669 +/- 110; O: 335 +/- 59 nM). Half-time for calcium influx was increased in the O group. P2X1 immunoreactivity per unit bladder weight was similar in the two groups. Cell membrane area per unit wet weight was decreased in the O group. Attenuated contractile responses to ATP in obstructed rat urinary b...Continue Reading

References

Oct 1, 1990·Cell and Tissue Research·G Gabella, B Uvelius
Jan 1, 1990·Anatomy and Embryology·G Gabella
Jan 1, 1985·General Pharmacology·G Burnstock, C Kennedy
Dec 1, 1987·British Journal of Urology·S C HarrisonP T Doyle
Aug 1, 1986·The Journal of Urology·J A GoslingC J Gilpin
Jan 1, 1994·World Journal of Urology·C H Hoyle
Mar 24, 1995·European Journal of Pharmacology·R SjuveB Uvelius
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·L VulchanovaR Elde
Jun 27, 2000·Cell and Tissue Research·H Y LeeG Burnstock
Dec 18, 2001·The Journal of Urology·Barry A O'ReillyStephen B McMahon

❮ Previous
Next ❯

Citations

Apr 29, 2008·Purinergic Signalling·Michael B HovaterErik M Schwiebert
Nov 23, 2013·Purinergic Signalling·Geoffrey Burnstock
May 17, 2011·Current Bladder Dysfunction Reports·Yan Sun, Toby C Chai
Sep 1, 2007·Scandinavian Journal of Urology and Nephrology·Anders ArnerBengt Uvelius
Oct 11, 2017·Frontiers in Pharmacology·Geoffrey Burnstock
Jul 31, 2021·Autonomic Neuroscience : Basic & Clinical·Christopher H Fry, Karen D McCloskey

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.