Changes in intracellular pH and pH regulating mechanisms in somitic cells of the early chick embryo: a study using fluorescent pH-sensitive dye

The Journal of Physiology
J I Gillespie, J R Greenwell

Abstract

1. Measurements of intracellular pH (pHi) have been made using the fluorescent dye dicyano-hydroquinone (DCH) in somites isolated from 2-day-old chick embryos. Measurements of pHi, from freshly dissected somites, gave values of 7.18 +/- 0.02 (mean +/- S.D., n = 12) while in somites kept in media containing 10% fetal calf serum for 2-5 h pHi was 7.36 +/- 0.02 (n = 11). 2. In freshly dissected control tissue recovery from an acid load (NH4Cl pre-pulse), in the nominal absence of HCO3-, was 0.007 +/- 0.003 pH units/min (n = 11). In the presence of 5 mM-HCO3- the rate of recovery was increased to 0.132 +/- 0.003 pH units/min (n = 11). This HCO3(-)-dependent recovery was inhibited by pre-treatment with DIDS (5 X 10(-4) M) and stopped when Na+ was replaced in the bathing medium with K+ or N-methylglucamine. Amiloride (10(-3) M) had no effect. 3. Replacement of Cl- with gluconate had little effect on pHi in control somites suggesting that absence of Cl(-)-HCO3- exchange. 4. These observations are consistent with the presence of a coupled Na+-HCO3- entry and the absence of Na+-H+ exchange. 5. In serum-treated cells recovery from an acid load was 0.101 +/- 0.01 pH units/min (n = 11) in the nominal absence of HCO3-. Increasing the HCO3- ...Continue Reading

Citations

Oct 1, 1990·Pflügers Archiv : European journal of physiology·K J Buckler, R D Vaughan-Jones
Mar 4, 2000·Journal of Neuroscience Research·A Boussouf, S Gaillard
Nov 1, 1993·Pflügers Archiv : European journal of physiology·J A NichollsJ R Greenwell
Jun 21, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Axel T EsserMichael Levin

Related Concepts

2,3-dicyanohydroquinone
Metazoa
Carbonic Acid Ions
Ampholytes
Chick Embryo
Fluorochromes
Hydrogen-Ion Concentration
Hydroquinones
Meglumine
Lateral Plate Mesoderm

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