Changes in lipid diffusibility in the hamster sperm head plasma membrane during capacitation in vivo and in vitro

Molecular Reproduction and Development
T T SmithD P Wolf

Abstract

The technique of fluorescence recovery after photobleaching (FRAP) was employed on spermatozoa labeled with the fluorescent lipid analogue C14dil to provide two measures of lateral diffusion in the plane of the sperm plasma membrane during capacitation in vivo and in vitro: the diffusion coefficient (D) for C14dil and the fraction of C14dil that is free to diffuse (%R) within the domain. To evaluate changes in lipid diffusibility during capacitation in vivo, spermatozoa were recovered from the uterus within 30 min after ejaculation or from the oviduct at 2, 4, 6 and 8 hr after mating. To compare the changes which occur in vivo with those which occur during capacitation in vitro, caudal epididymal spermatozoa were incubated under capacitating or non-capacitating (control) conditions for 4 hr. Although transient changes in D occurred during the course of capacitation, there was no net change in D for either anterior (AH) or posterior head (PH) domains following capacitation in vitro or in vivo. Significant differences in the lipid diffusion coefficient between the two head domains were observed during the course of capacitation. A transient decrease in %R was observed for the AH domain during capacitation in vitro and incubation ...Continue Reading

References

Aug 10, 1979·Science·S NagaseS Shumiya
Dec 1, 1990·Journal of Electron Microscopy Technique·E L Bearer, D S Friend
May 1, 1989·Journal of Reproduction and Fertility·T T Smith, R Yanagimachi
Mar 1, 1988·Journal of Reproduction and Fertility·H J Leese
Jan 1, 1987·Annals of the New York Academy of Sciences·D E Wolf
Oct 1, 1986·Journal of Ultrastructure and Molecular Structure Research·J Tesarík, J E Fléchon
Jun 1, 1986·Biology of Reproduction·R A Cardullo, R A Cone
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·B K DavisK Bedigian
May 1, 1984·The Journal of Cell Biology·D E Wolf, J K Voglmayr
Mar 1, 1982·The Journal of Cell Biology·E L Bearer, D S Friend
May 1, 1982·The Journal of Cell Biology·D S Friend
Oct 1, 1994·Molecular Reproduction and Development·R H Hunter
Sep 1, 1979·Biophysical Chemistry·B G Barisas, M D Leuther

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Citations

Feb 28, 2003·Biology of Reproduction·Matthew R Nimmo, Nicholas L Cross
Apr 18, 2003·Biology of Reproduction·Nicholas L Cross
Oct 1, 2004·Molecular Reproduction and Development·Steve TardifJanice L Bailey
Oct 16, 2004·Biology of Reproduction·Natali Anne Henderson, Bernard Robaire
Mar 3, 2006·Biology of Reproduction·Hanae RejrajiJoël R Drevet
Aug 10, 2006·The Journal of Clinical Endocrinology and Metabolism·R John AitkenEileen A McLaughlin
Apr 15, 2020·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Imran TariqueQuisheng Chen
Jan 30, 2016·Molecular Reproduction and Development·Charoonroj ChotwiwatthanakunRapeepun Vanichviriyakit
Sep 21, 2010·Systems Biology in Reproductive Medicine·Janice L Bailey

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