Changes in metabolism and hormone trafficking during exposure of endocrine cells to elevated ammonium.

Cytotechnology
J J Dyken-Young, A Sambanis

Abstract

Endocrine cell cultures have potential in bioprocessing, for the production of biologically active hormones, and in tissue engineering, for the development of implantable artificial tissues for long-term restoration of endocrine function. To optimize such systems, it is necessary to develop a thorough understanding of how inherently present environmental stresses, such as nutrient depletion and metabolite accumulation, affect the cells. This work focuses on the effects of the metabolite ammonium on indicators of endocrine cell metabolism and on the processing, storage and secretion of regulated secretory proteins. Experiments were conducted on recombinant insulin-producing mouse pituitary AtT-20 cells and mouse insulinoma βTC3 cells. Exposure for 24-48 hours to 6 mM of exogenous ammonium resulted in higher rates of glucose consumption by both AtT-20 and βTC3 cells, while the formation of additional ammonium generally decreased relative to ammonium-free controls. When βTC3 cells were discharged of their intracellular insulin stores, the presence of ammonium during a subsequent recharge completely inhibited addition of new insulin-related peptides to the stores, as we had observed previously for both cell lines. There was a corre...Continue Reading

References

Oct 13, 1992·Annals of the New York Academy of Sciences·G E Grampp, G Stephanopoulos
Jan 1, 1986·Annual Review of Physiology·R C De Lisle, J A Williams
Dec 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S EfratS Baekkeskov
Jan 13, 1989·Science·K N ChungH P Moore
Jan 1, 1988·Ciba Foundation Symposium
Feb 1, 1994·Enzyme and Microbial Technology·J J Dyken, A Sambanis
Aug 5, 1988·Biotechnology and Bioengineering·M W GlackenA J Sinskey
May 1, 1990·Biotechnology and Bioengineering·A McQueen, J E Bailey

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