Changes in Metabolites Present in Lung-Lining Fluid Following Exposure of Humans to Ozone

Toxicological Sciences : an Official Journal of the Society of Toxicology
WanYun ChengR B Devlin

Abstract

Controlled human exposure to the oxidant air pollutant ozone causes decrements in lung function and increased inflammation as evidenced by neutrophil influx into the lung and increased levels of proinflammatory cytokines in the airways. Here we describe a targeted metabolomics evaluation of human bronchoalveolar lavage fluid (BALF) following controlled in vivo exposure to ozone to gain greater insight into its pulmonary effects. In a 2-arm cross-over study, each healthy adult human volunteer was randomly exposed to filtered air (FA) and to 0.3 ppm ozone for 2 h while undergoing intermittent exercise with a minimum of 4 weeks between exposures. Bronchoscopy was performed and BALF obtained at 1 (n = 9) or 24 (n = 23) h postexposure. Metabolites were detected using ultrahigh performance liquid chromatography-tandem mass spectroscopy. At 1-h postexposure, a total of 28 metabolites were differentially expressed (DE) (p < .05) following ozone exposure compared with FA-exposure. These changes were associated with increased glycolysis and antioxidant responses, suggesting rapid increased energy utilization as part of the cellular response to oxidative stress. At 24-h postexposure, 41 metabolites were DE. Many of the changes were in ami...Continue Reading

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Citations

Aug 14, 2019·Reviews on Environmental Health·Pan DengBernhard Hennig
Sep 29, 2020·Toxicological Sciences : an Official Journal of the Society of Toxicology·Mary FrancisDebra L Laskin
May 21, 2020·Frontiers in Veterinary Science·Gabrielle BrankstonJanet Beeler-Marfisi
Jul 30, 2019·Frontiers in Immunology·Sharon MumbyIan M Adcock
Jan 10, 2021·Current Environmental Health Reports·Lan JinYawei Zhang

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