Changes in microRNAs associated with Twist-1 and Bcl-2 overexpression identify signaling pathways

Experimental and Molecular Pathology
Nan ZhaoQiang Gu

Abstract

Epithelial-mesenchymal transition (EMT) is regulated by multiple signal transduction pathways. Twist-1 is one of the most important transcription factors in these pathways. In a previous study, we found that Bcl-2 enhanced the role of Twist-1 in EMT. Coexpression of Twist-1 and Bcl-2 may play an import role in vasculogenic mimicry (VM) through regulation of EMT. Moreover, regulators of EMT and VM are known to be important targets for microRNAs (miRNAs). To better understand how these critical pathways are induced by coexpression of Twist-1 and Bcl-2, we performed a comprehensive comparative bioinformatics analysis using microarrays on HCCs that overexpressed Twist-1 and Bcl-2. Eleven miRNAs associated with coexpression of Twist-1 and Bcl-2 were selected from the comprehensive analysis of miRNA microarray and ChIP-seq analysis. Changes in miRNAs were associated with significant differences in the expression of genes involved in signal transduction pathways related to processes including tumor invasion, metastasis, angiogenesis, and tumor cell shape. We confirmed the role of Twist-1 and Bcl-2 coexpression in HCC cells using wound healing assays, invasion assays, and 3D Matrigel assays. Furthermore, the role of miR-27a as a crucia...Continue Reading

References

Sep 17, 1999·The American Journal of Pathology·A J ManiotisM J Hendrix
Dec 6, 2003·Genome Research·Thomas SchlittAlvis Brazma
Jun 10, 2005·Nature·Jun LuTodd R Golub
Dec 31, 2005·Nucleic Acids Research·UNKNOWN Gene Ontology Consortium
Feb 8, 2006·Proceedings of the National Academy of Sciences of the United States of America·Stefano VoliniaCarlo M Croce
Jan 24, 2007·Journal of Cellular Biochemistry·Joyce C Tse, Raghu Kalluri
Dec 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Xu-Bao ShiRalph W deVere White
Oct 29, 2008·Oncogene·K W Yip, J C Reed
Dec 4, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tao SunBao-Cun Sun
Jan 19, 2010·Biochimica Et Biophysica Acta·Yvette W J PaulisArjan W Griffioen
Feb 4, 2010·Cancer Research·Xiaoxia HuXiaowei Wang
Jul 13, 2011·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Tao SunZhi-yong Liu
May 26, 2012·Clinical and Experimental Medicine·Amalia VartanianEugenia Stepanova
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marzia RossatoFlavia Bazzoni
Apr 2, 2015·Biochimica Et Biophysica Acta·Cristina CorreiaScott H Kaufmann

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Citations

Oct 27, 2015·International Journal of Molecular Medicine·Wei-Lu WuGan-Di Li
May 3, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shuixian LiXiuzhen Han
Nov 26, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Qing-Qing ZhuTangfeng Lv
Nov 8, 2017·International Journal of Molecular Sciences·Ana CavacoJohannes A Eble
Dec 14, 2018·International Journal of Molecular Sciences·Tae-Su HanHyun-Soo Cho
Feb 22, 2021·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Ning ZhengJichuang Wang

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