Changes in mitochondrial morphology induced by calcium or rotenone in primary astrocytes occur predominantly through ros-mediated remodeling

Journal of Neurochemistry
Samineh DeheshiGordon L Rintoul

Abstract

Morphological changes in mitochondria have been primarily attributed to fission and fusion, while the more pliable transformations of mitochondria (remodeling, rounding, or stretching) have been largely overlooked. In this study, we quantify the contributions of fission and remodeling to changes in mitochondrial morphology induced by the Ca(2+) ionophore 4Br-A23187 and the metabolic toxin rotenone. We also examine the role of reactive oxygen species (ROS) in the regulation of mitochondrial remodeling. In agreement with our previous studies, mitochondrial remodeling, not fission, is the primary contributor to Ca(2+) -mediated changes in mitochondrial morphology induced by 4Br-A23187 in rat cortical astrocytes. Treatment with rotenone produced similar results. In both paradigms, remodeling was selectively blocked by antioxidants whereas fission was not, suggesting a ROS-mediated mechanism for mitochondrial remodeling. In support of this hypothesis, inhibition of endogenous ROS by overnight incubation in antioxidants resulted in elongated reticular networks of mitochondria. Examination of inner and outer mitochondrial membranes revealed that they largely acted in concert during the remodeling process. While mitochondrial morpholog...Continue Reading

References

Feb 15, 1994·Microscopy Research and Technique·J Bereiter-Hahn, M Vöth
Mar 4, 1997·Proceedings of the National Academy of Sciences of the United States of America·L A HerzenbergL A Herzenberg
May 2, 1998·Brain Research·T YamasobaJ M Miller
Sep 30, 1998·Journal of Neuroscience Research·J M Dubinsky, Y Levi
Mar 18, 1999·Biochemical and Biophysical Research Communications·A A BoldyrevP Johnson
Dec 2, 2000·Nature Neuroscience·R BetarbetJ T Greenamyre
Oct 14, 2003·Journal of Korean Medical Science·Sae-Yong HongZee-Won Lee
Jan 15, 2004·The Journal of Biological Chemistry·George T HansonS James Remington
Aug 5, 2004·Annals of Neurology·Giovanni Manfredi, M Flint Beal
Sep 10, 2004·American Journal of Physiology. Cell Physiology·Paul S BrookesShey-Shing Sheu
May 19, 2005·The Journal of Biological Chemistry·Hsiuchen ChenDavid C Chan
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Richard J Youle, Mariusz Karbowski
Mar 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Rabindra TirouvanziamLeonard A Herzenberg
Apr 5, 2007·Nature Protocols·Stefanie Kaech, Gary Banker
Apr 25, 2007·Apoptosis : an International Journal on Programmed Cell Death·Eric C C CheungRuth S Slack
Jun 1, 2007·The Journal of Biological Chemistry·Arezu Jahani-AslRuth S Slack
Jun 8, 2007·The Journal of Biological Chemistry·Chuang-Rung Chang, Craig Blackstone
Oct 2, 2007·EMBO Reports·Arezu Jahani-Asl, Ruth S Slack
Oct 12, 2007·Nature Reviews. Molecular Cell Biology·Scott A Detmer, David C Chan
Feb 23, 2008·The International Journal of Biochemistry & Cell Biology·Andrea DlaskováPetr Jezek
Mar 15, 2008·Journal of Interferon & Cytokine Research : the Official Journal of the International Society for Interferon and Cytokine Research·Ariel C MillmanVishwanath Venketaraman
Apr 17, 2008·Journal of Medicinal Chemistry·Sharon KempJanice R Aldrich-Wright
Apr 26, 2008·Antioxidants & Redox Signaling·Giovanni Benard, Rodrigue Rossignol
Jun 19, 2008·Genes & Development·Der-Fen SuenRichard J Youle
Sep 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hansong DengMing Guo
Oct 8, 2008·Proceedings of the National Academy of Sciences of the United States of America·G M CereghettiL Scorrano
Nov 18, 2008·Proceedings of the National Academy of Sciences of the United States of America·Jyoti D MalhotraRandal J Kaufman
Jan 10, 2009·The Journal of Pharmacology and Experimental Therapeutics·Zhaosheng HanB Rita Alevriadou
Sep 15, 2009·Biochimica Et Biophysica Acta·Gordon L Rintoul, Ian J Reynolds
Oct 8, 2009·Human Molecular Genetics·Hsiuchen Chen, David C Chan
Jan 1, 2010·Molecular Neurodegeneration·Shamim B Shaikh, Louise Fb Nicholson
Mar 30, 2010·Biochimica Et Biophysica Acta·Jennifer HomShey-Shing Sheu

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Citations

Jan 16, 2016·Molecular and Cellular Neurosciences·Diepiriye G IworimaGordon L Rintoul
Aug 22, 2015·Redox Biology·Agnes GörlachOlga Krizanova
Feb 22, 2018·Ultrastructural Pathology·Sanaa A M ElgayarRaghda Elsherif
May 16, 2018·Reproduction, Fertility, and Development·Abdul HaseebQiusheng Chen
Dec 12, 2018·Frontiers in Endocrinology·Jordan RowlandsRodrigo Carlessi
May 18, 2019·Journal of Diabetes Research·Janet Ya-An ChangGladys Y-P Ko
Feb 13, 2017·Neurochemical Research·Hai-Ning LiP Andy Li
Jun 9, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhendong ZhaoZiqian Li
Jan 10, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Alexandra C E DraperRichard J Piercy
Aug 19, 2020·Redox Biology·Corina T Madreiter-SokolowskiMichael Ristow

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