PMID: 6402774Feb 1, 1983Paper

Changes in mitochondrial structure and function in 9l rat brain tumor cells treated in vitro with alpha-difluoromethylornithine, a polyamine biosynthesis inhibitor

Proceedings of the National Academy of Sciences of the United States of America
S M OredssonL J Marton

Abstract

Mitochondrial structure and function were studied in 9L rat brain tumor cells depleted of polyamines by alpha-difluoromethylornithine, an enzyme-activated irreversible inhibitor of ornithine decarboxylase. Cells treated with methylglyoxal bis(guanylhydrazone), a reversible inhibitor of S-adenosylmethionine decarboxylase, were used for comparison because this polyamine biosynthesis inhibitor is known to cause structural and functional disruption of mitochondria. A significant increase in mitochondrial size, measured quantitatively, was found in alpha-difluoromethylornithine-treated cells (10 mM for 72 hr) compared with untreated cells (P < 0.001). This increase in mitochondrial size was reversed when putrescine was added to the cultures for 24 hr after alpha-difluoromethylornithine treatment. Putrescine alone had no effect on the size of mitochondria. Treatment of cells with methylglyoxal bis(guanylhydrazone) (80 muM for 48 hr) caused only a slight increase in mitochondrial size compared with mitochondria in untreated cells (P < 0.05) and failed to produce the dramatic ultrastructural changes reported in other cell lines. Ultrastructural examination revealed an increase in cytoplasmic and membrane-associated ribosomes in alpha-d...Continue Reading

References

Oct 12, 1978·Biochimica Et Biophysica Acta·G QuarfothD Foster
Jan 30, 1979·Biochemical and Biophysical Research Communications·R R ChaffeeR H Rochelle
Jul 1, 1979·Somatic Cell Genetics·G A Breen, I E Scheffler
Jan 1, 1978·The International Journal of Biochemistry·H W PeterT Günther
Oct 23, 1970·Biochemical and Biophysical Research Communications·J A Khawaja, A Raina
Mar 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·M SchindlerM P Sheetz
Jun 1, 1981·Journal of Cellular Physiology·J J HaradaD R Morris

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Citations

Dec 1, 1987·Photochemistry and Photobiology·D P ValenzenoM Milne

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