Changes in morphogen kinetics and pollen grain size are potential mechanisms of aberrant pollen aperture patterning in previously observed and novel mutants of Arabidopsis thaliana

PLoS Computational Biology
Shayne M PlourdeAnna A Dobritsa

Abstract

Pollen provides an excellent system to study pattern formation at the single-cell level. Pollen surface is covered by the pollen wall exine, whose deposition is excluded from certain surface areas, the apertures, which vary between the species in their numbers, positions, and morphology. What determines aperture patterns is not understood. Arabidopsis thaliana normally develops three apertures, equally spaced along the pollen equator. However, Arabidopsis mutants whose pollen has higher ploidy and larger volume develop four or more apertures. To explore possible mechanisms responsible for aperture patterning, we developed a mathematical model based on the Gierer-Meinhardt system of equations. This model was able to recapitulate aperture patterns observed in the wild-type and higher-ploidy pollen. We then used this model to further explore geometric and kinetic factors that may influence aperture patterns and found that pollen size, as well as certain kinetic parameters, like diffusion and decay of morphogens, could play a role in formation of aperture patterns. In conjunction with mathematical modeling, we also performed a forward genetic screen in Arabidopsis and discovered two mutants with aperture patterns that had not been ...Continue Reading

References

Aug 1, 1990·The Plant Cell·D R SmythE M Meyerowitz
Dec 1, 1972·Kybernetik·A Gierer, H Meinhardt
Sep 15, 1998·Journal of Theoretical Biology·A RessayreP H Gouyon
Apr 24, 2002·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·C VareaR A Barrio
Mar 9, 2004·Trends in Plant Science·Carol A Furness, Paula J Rudall
Sep 22, 2007·Bulletin of Mathematical Biology·R A BarrioP K Maini
Sep 17, 2008·Essays in Biochemistry·Brian Ingalls
Apr 11, 2009·Proceedings of the National Academy of Sciences of the United States of America·Diane M MartinJörg Bohlmann
Jun 11, 2009·PLoS Biology·Isabelle d'ErfurthRaphaël Mercier
Apr 21, 2010·Proceedings of the National Academy of Sciences of the United States of America·Eleni KatiforiJacques Dumais
Aug 8, 2015·The New Phytologist·Alexis Matamoro-VidalPierre-Henri Gouyon
Jan 20, 2018·Physical Review. E·Heather A Brooks, Paul C Bressloff

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Citations

Feb 6, 2020·Journal of Experimental Botany·Batthula Vijaya Lakshmi Vadde, Adrienne H K Roeder
Sep 30, 2020·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Asja Radja
Apr 15, 2020·Nature Plants·Yuan Zhou, Anna A Dobritsa
Sep 16, 2019·Plant Science : an International Journal of Experimental Plant Biology·Yuan Zhou, Anna A Dobritsa
Jun 30, 2021·Nature Plants·Byung Ha LeeAnna A Dobritsa
Jul 27, 2021·Frontiers in Plant Science·Ismael Mazuecos-AguileraVíctor N Suárez-Santiago

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Methods Mentioned

BETA
dissecting
confocal microscopy

Software Mentioned

FlexPDE

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