Changes in morphogenesis and carotenogenesis to influence polygalacturonase secretion in Aspergillus carbonarius mutant.

Archives of Microbiology
Ahila Mathimaran, Anbarasu Kumar

Abstract

Mycelial morphogenesis and the production of fungal secretory proteins are still largely unknown. A mutant strain of Aspergillus carbonarius UV-10046 produced abundant polygalacturonase (PG) along with partially saturated canthaxanthin (PSC) at low pH conditions. In the present study, the relationship between PG secretion and PSC biosynthesis was studied using carotenogenic inhibitors and SDS-PAGE electrophoresis. Also the correlation between morphogenesis and PG secretion was investigated by analysing through microscopic studies. From the results, it was observed that secretion of PG was positively influenced by the PSC biosynthesis. The results also showed that the mutant with hairy mycelial structure resulted in higher PG activity when compared to the wild type that lacks hyper branching. From the results, it was confirmed that a mutation might have occurred in the isoprenoid pathway that has helped mutant for survival at acidic conditions. Further, an alteration in the morphogenesis and hyper branching development caused over secretion of PG enzyme in the mutant.

References

Aug 1, 1991·Journal of General Microbiology·H A WöstenJ G Wessels
Dec 29, 1998·Analytical Biochemistry·C SureshS Umesh-Kumar
Jul 3, 1999·Applied Microbiology and Biotechnology·C SureshN G Karanth
Dec 23, 1999·Current Opinion in Microbiology·X Xiang, N R Morris
Sep 23, 2000·Journal of Microbiological Methods·C L GordonG D Robson
Mar 28, 2002·Applied and Environmental Microbiology·Christian MüllerJens Nielsen
Dec 11, 2003·Biotechnology Advances·Maria Papagianni
May 24, 2005·Applied Microbiology and Biotechnology·R te BiesebekeC A M J J van den Hondel
Jun 11, 2008·Applied Microbiology and Biotechnology·Nallasamy KumaresanSukumaran Umesh-Kumar
Apr 25, 1990·Biotechnology and Bioengineering·J J SmithR I Fox
Apr 15, 1990·Biotechnology and Bioengineering·S E Vecht-LifshitzS Braun
Jul 14, 2009·Bioresource Technology·Aftab Ahamed, Patrick Vermette
Dec 20, 2014·BMC Genomics·Tatiana Q AguiarLucília Domingues

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis

Software Mentioned

SPSS
Magvision

Related Concepts

Related Feeds

Attention Disorders

Attention is involved in all cognitive activities, and attention disorders are reported in patients with various neurological diseases. Here are the latest discoveries pertaining to attention disorders.