Changes in morphology and optical properties of sclera and choroidal layers due to hyperosmotic agent

Journal of Biomedical Optics
Raiyan T ZamanAshley J Welch


Light scattering in the normally white sclera prevents diagnostic imaging or delivery of a focused laser beam to a target in the underlying choroid layer. In this study, we examine optical clearing of the sclera and changes in blood flow resulting from the application of glycerol to the sclera of rabbits. Recovery dynamics are monitored after the application of saline. The speed of clearing for injection delivery is compared to the direct application of glycerol through an incision in the conjunctiva. Although, the same volume of glycerol was applied, the sclera cleared much faster (5 to 10 s) with the topical application of glycerol compared to the injection method (3 min). In addition, the direct topical application of glycerol spreads over a larger area in the sclera than the latter method. A diffuse optical spectroscopy system provided spectral analysis of the remitted light every two minutes during clearing and rehydration. Comparison of measurements to those obtained from phantoms with various absorption and scattering properties provided estimates of the absorption coefficient and reduced scattering coefficient of rabbit eye tissue.


Nov 1, 1975·The British Journal of Ophthalmology·S S Hayreh
Jan 1, 1987·Lasers in Surgery and Medicine·A J WelchM J van Gemert
Aug 1, 1994·The Journal of Clinical Investigation·S M WahlJ B McCarthy
Jan 1, 1996·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·K NishimuraS Mita
Jan 1, 1997·Journal of the Optical Society of America. A, Optics, Image Science, and Vision·A Kienle, M S Patterson
May 1, 1997·Physics in Medicine and Biology·A R Young
Oct 21, 1998·Experimental Eye Research·Y ZhuJ M Gidday
Apr 1, 1999·Lasers in Surgery and Medicine·G VargasA J Welch
Nov 30, 2000·Experimental Eye Research·J W Kiel
Aug 15, 2002·Journal of Biomedical Optics·Zachary S SacksGerard A Mourau
Jul 17, 2004·Annual Review of Biomedical Engineering·C Ross EthierJeff Ruberti
Dec 6, 2006·The Journal of Veterinary Medical Science·Hiroyoshi Ninomiya, Tomo Inomata
May 10, 2008·Journal of Biomedical Optics·Mohamad G GhosnKirill V Larin
Sep 6, 2008·Optometry and Vision Science : Official Publication of the American Academy of Optometry·Yunwei Feng, Trefford L Simpson
Nov 22, 2008·Journal of Biomedical Optics·Narasimhan RajaramJames W Tunnell
Jan 10, 2009·Experimental and Toxicologic Pathology : Official Journal of the Gesellschaft Für Toxikologische Pathologie·T Steger-HartmannJ Walter
Aug 12, 2009·Lasers in Surgery and Medicine·Raiyan T ZamanAshley J Welch
Jun 15, 2011·Analytical Biochemistry·William A HayesLisa M Collins
Oct 1, 1997·Journal of Biomedical Optics·V V TuchinA A Mishin
Jan 1, 1999·Journal of Biomedical Optics·V V Tuchin
Nov 1, 2007·IEEE Journal of Selected Topics in Quantum Electronics : a Publication of the IEEE Lasers and Electro-optics Society·Raiyan T ZamanJames W Tunnell

❮ Previous
Next ❯


Dec 1, 2011·Lasers in Surgery and Medicine·Raiyan T ZamanHenry G Rylander
Mar 22, 2013·Journal of Biomedical Optics·Omid MasihzadehTim C Lei
Aug 15, 2020·Experimental Biology and Medicine·Ruiming KongCuixia Dai

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Imaging in CNS

Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.