Light scattering in the normally white sclera prevents diagnostic imaging or delivery of a focused laser beam to a target in the underlying choroid layer. In this study, we examine optical clearing of the sclera and changes in blood flow resulting from the application of glycerol to the sclera of rabbits. Recovery dynamics are monitored after the application of saline. The speed of clearing for injection delivery is compared to the direct application of glycerol through an incision in the conjunctiva. Although, the same volume of glycerol was applied, the sclera cleared much faster (5 to 10 s) with the topical application of glycerol compared to the injection method (3 min). In addition, the direct topical application of glycerol spreads over a larger area in the sclera than the latter method. A diffuse optical spectroscopy system provided spectral analysis of the remitted light every two minutes during clearing and rehydration. Comparison of measurements to those obtained from phantoms with various absorption and scattering properties provided estimates of the absorption coefficient and reduced scattering coefficient of rabbit eye tissue.
A diffusion theory model of spatially resolved, steady-state diffuse reflectance for the noninvasive determination of tissue optical properties in vivo
Synthetic fibronectin peptides suppress arthritis in rats by interrupting leukocyte adhesion and recruitment
A comparison of two methods of measuring aqueous flow in humans: fluorophotometry and flare measurement
Improved solutions of the steady-state and the time-resolved diffusion equations for reflectance from a semi-infinite turbid medium
Use of osmotically active agents to alter optical properties of tissue: effects on the detected fluorescence signal measured through skin
Morphological changes in blood vessels produced by hyperosmotic agents and measured by optical coherence tomography
Effect of pigment packaging on diffuse reflectance spectroscopy of samples containing red blood cells
Diffuse-reflectance spectroscopy from 500 to 1060 nm by correction for inhomogeneously distributed absorbers
Predictions and measurements of scattering and absorption over broad wavelength ranges in tissue phantoms
Differential permeability rate and percent clearing of glucose in different regions in rabbit sclera
The involvement of pro-inflammatory cytokines in nephrogenic systemic fibrosis - a mechanistic hypothesis based on preclinical results from a rat model treated with gadodiamide
Experimental validation of the effects of microvasculature pigment packaging on in vivo diffuse reflectance spectroscopy
Lookup-table-based inverse model for mapping oxygen concentration of cutaneous microvessels using hyperspectral imaging
Imaging depth extension of optical coherence tomography in rabbit eyes using optical clearing agents.
Lookup-table-based inverse model for human skin reflectance spectroscopy: two-layered Monte Carlo simulations and experiments
Cell Imaging in CNS
Here is the latest research on cell imaging and imaging modalities, including light-sheet microscopy, in the central nervous system.