Changes in non-core regions stabilise plastocyanin from the thermophilic cyanobacterium Phormidium laminosum.

Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry
Francisco J Muñoz-LópezPaolo Carloni

Abstract

We report a theoretical investigation on the different stabilities of two plastocyanins. The first one belongs to the thermophilic cyanobacterium Phormidium laminosum and the second one belongs to its mesophilic relative Synechocystis sp. These proteins share the same topology and secondary-structure elements; however, the melting temperatures of their oxidised species differ by approximately 15 K. Long-time-scale molecular dynamics simulations, performed at different temperatures, show that the thermophilic protein optimises a set of intramolecular interactions (interstrand hydrogen bonding, salt bridging and hydrophobic clustering) within the region that comprises the strands beta 5 and beta 6, loop L5 and the helix. This region exhibits most of the differences in the primary sequence between the two proteins and, in addition, it is involved in the interaction with known physiological partners. Further work is in progress to unveil the specific structural features responsible for the different thermal stability of the two proteins.

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Citations

Apr 20, 2010·FEBS Letters·Francisco J Muñoz-LópezAntonio Díaz-Quintana
Feb 1, 2011·Chemistry & Biology·Jesús ChaboyAntonio Díaz-Quintana
Jul 11, 2013·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Stefania EvoliBruno Rizzuti

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