Changes in nuclear localization of An3, a RNA helicase, during oogenesis and embryogenesis in Xenopus laevis

Molecular Reproduction and Development
F J LongoD L Weeks

Abstract

The immunolocalization of An3 protein, an ATP-dependent RNA helicase and a member of the DEAD box family, was compared with the localization of fibrillarin, a protein essential for rRNA processing, and snRNPs, which are involved in mRNA splicing reactions, during oogenesis and embryogenesis in Xenopus laevis. Although An3 protein was detected in the cytoplasm of all stages of oocytes, in most stages An3 protein was also present in the nucleus. Prior to stage I An3 protein was uniformly dispersed throughout the entire germinal vesicle; from stages I to V it was in nucleoli. By stage VI nucleolar labeling with anti-An3 disappeared and the protein was no longer present within nuclei. An3 reactivity was also present throughout the nuclei of follicle cells surrounding prestage I to stage VI oocytes. Both cytoplasmic and nuclear An3 staining were present in cells of stages 8 to 35 embryos; however, nuclear staining was punctate and uniformly distributed throughout the nucleoplasm. Fibrillarin was diffusely distributed throughout the entire germinal vesicle prior to stage I, localized exclusively to nucleoli of oocytes between stages I and VI and in nucleoli of stages 12 and 35 embryonic cells. Reactivity for snRNPs (anti-Sm) in germi...Continue Reading

References

Apr 1, 1992·The Journal of Cell Biology·M Carmo-FonsecaA I Lamond
Feb 1, 1992·Molecular Microbiology·S R Schmid, P Linder
Jun 25, 1992·Nucleic Acids Research·L F PattersonP F Lasko
Jul 10, 1992·Cell·U T Meier, G Blobel
Nov 22, 1991·Proceedings. Biological Sciences·K T SillarA J Simmers
Feb 7, 1991·Nature·D A Wassarman, J A Steitz
Jan 1, 1991·Genes & Development·H Ellinger-Ziegelbauer, C Dreyer
Mar 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T de ValoirK Beckingham
Jan 1, 1990·Electron Microscopy Reviews·I RaskaL Salamin-Michel
Sep 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·J G Gall, H G Callan
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S LiM G Rosenfeld
Jan 12, 1989·Nature·P LinderP P Slonimski
Dec 1, 1981·The Journal of Cell Biology·O L Miller
Jun 1, 1994·Current Opinion in Cell Biology·U Scheer, D Weisenberger
Mar 15, 1994·Proceedings of the National Academy of Sciences of the United States of America·R GururajanD L Weeks
Aug 13, 1993·Cell·M S Schmidt-ZachmannE A Nigg
Jun 1, 1993·Current Opinion in Cell Biology·M McKeown

❮ Previous
Next ❯

Citations

Jul 17, 1998·The Journal of Pathology·U A Heinlein
Sep 28, 2005·Histochemistry and Cell Biology·Vladimír BaranJan Motlík
Apr 15, 2000·The Journal of Biological Chemistry·P AskjaerJ Kjems
Aug 24, 1999·Molecular and Cellular Biology·P AskjaerM Fornerod
Jan 31, 2003·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Michael W KingAnton W Neff
Dec 2, 2004·Developmental Biology·Oona JohnstonePaul Lasko

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.