Abstract
Previous investigations showed that inhibition of DNA synthesis by hydroxyurea, aphidicolin, or 5-fluorodeoxyuridine produced large changes in the composition and nucleosome repeat lengths of bulk chromatin. Here we report results of investigations to determine whether the changes in nucleosome repeat lengths might be localized in the initiated replicons, as postulated [D'Anna, J. A., & Prentice, D. A. (1983) Biochemistry 22, 5631-5640]. In most experiments, Chinese hamster (line CHO) cells were synchronized in G1, or they were synchronized in early S phase by allowing G1 cells to enter S phase in medium containing 1 mM hydroxyurea or 5 micrograms mL-1 aphidicolin, a procedure believed to produce an accumulation of initiated replicons that arise from normally early replicating DNA. Measurements of nucleosome repeat lengths of bulk chromatin, the early replicating unexpressed metallothionein II (MTII) gene region, and a later replicating repeated sequence indicate that the changes in repeat lengths occur preferentially in the early replicating MTII gene region as G1 cells enter and become synchronized in early S phase. During that time, the MTII gene region is not replicated nor is there any evidence for induction of MTII messen...Continue Reading
References
Dec 1, 1977·Cancer·J D CrissmanA Harris
Apr 1, 1977·Cell·J O Thomas, R J Thompson
Oct 18, 1977·Biochemistry·H LehrachH Boedtker
Sep 20, 1976·Biochimica Et Biophysica Acta·R A WaltersC E Hildebrand
Nov 5, 1975·Journal of Molecular Biology·E M Southern
Mar 8, 1976·Biochemical and Biophysical Research Communications·R A WaltersC E Hildebrand
Sep 1, 1986·Somatic Cell and Molecular Genetics·R L StallingsC E Hildebrand
Sep 10, 1985·Biochemistry·J A D'AnnaR Tobey
Feb 1, 1985·Molecular and Cellular Biology·B D CrawfordR A Walters
Nov 1, 1986·Molecular and Cellular Biology·R S DeckerM L DePamphilis
Oct 1, 1986·Molecular and Cellular Biology·R N JohnstonR T Schimke
Jan 1, 1988·Chromosoma·M A CaligoG Rainaldi
May 1, 1988·Molecular and Cellular Biology·N H Heintz, B W Stillman
Sep 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·R K MoyzisJ R Wu
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R T SchimkeR N Johnston
Sep 23, 1986·Biochemistry·M L MorenoJ L Stein
Dec 18, 1986·Biochimica Et Biophysica Acta·J A D'AnnaR A Tobey
Jan 26, 1987·Nucleic Acids Research·J L LongmireC E Hildebrand
Oct 25, 1985·Nucleic Acids Research·K C Reed, D A Mann
Feb 1, 1974·The Journal of Cell Biology·P N Dean, J H Jett
May 1, 1984·Analytical Biochemistry·J Meinkoth, G Wahl
Oct 9, 1984·Biochemistry·J A D'Anna, R A Tobey
Apr 1, 1981·Analytical Biochemistry·G DretzenP Chambon
Jan 1, 1984·Chromosoma·G RainaldiT Mariani
Oct 1, 1984·Molecular and Cellular Biology·R A Tobey, J Seagrave
Nov 22, 1983·Biochemistry·J A D'Anna, D A Prentice
Jun 1, 1984·Molecular and Cellular Biology·T D TlstyR T Schimke
Aug 25, 1983·Nucleic Acids Research·I M Leffak
Jul 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·N H Heintz, J L Hamlin
Aug 17, 1982·Biochemistry·J A D'AnnaR A Tobey
May 1, 1981·Journal of Toxicology and Environmental Health·M D EngerC E Hildebrand
Dec 1, 1980·Biological Trace Element Research·C E HildebrandR A Tobey
Citations
Jan 27, 1998·Journal of Cellular Biochemistry·J LaitinenE Hölttä
Jan 1, 1995·Journal of Cellular Biochemistry·J LaitinenE Hölttä
Apr 1, 1995·Journal of Cellular Biochemistry·J LaitinenE Hölttä
Mar 20, 1992·Journal of Molecular Biology·J M GaleJ A D'Anna
May 21, 1993·Cell·V L MakarovJ P Langmore
Aug 17, 2010·Journal of Molecular Biology·Ognjen PerišićTamar Schlick
Jul 25, 1997·Journal of Molecular Biology·K Liu, A Stein
Feb 11, 1992·Biochemistry·B VilleponteauH G Martinson
May 15, 1990·Analytical Biochemistry·J A D'AnnaR A Tobey