PMID: 9185462May 1, 1997Paper

Changes in opioid receptor binding nature in rat brain and spinal cord following formalin or carrageenan-induced nociception

Masui. The Japanese journal of anesthesiology
S OhtaS Dohi

Abstract

Previous reports demonstrated the regulation of opioid and their receptor in nociception, but it is not clear how nociceptive activation may alter opioid receptor binding nature. We determined the change of mu, delta and kappa opioid receptor types in brain and spinal cord homogenates obtained from animal models receiving nociceptive treatment. 1) Rats received a subcutaneous injection of formalin and carrageenan into planter aspect of a hindpaw. These agent-injected animals were observed for appearance of pain-related behavior (guarding of the treated paw) within 2-3 hour after treatment. Following these pain behavior, rats were decapitated and brain and spinal cord were removed rapidly. 2) The binding of 3H-DAGO (mu agonist), 3H-DPDPE (delta agonist) and 3H-EKC (kappa agonist) to brain and spinal cord membranes prepared from nociceptive treatment and control rats was determined. Using these tracer 3H-opioid ligands, we failed to see any change in the total number of opioid binding sites (Bmax values) or the affinity constant (Kd values) for binding in whole brain and spinal cord. These results indicate that in these animal models which use experimentally induced inflammation to stimulate a condition of nociception, there appe...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.